Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana

Takashi Hirayama, Imajuku Yoshiro, Anai Toyoaki, Matsui Minami, Oka Atsuhiro

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

The cdc2 gene product (p34cdc2) has been thought to play a central role in control of the mitotic cell cycle of yeasts and animals. To approach an understanding of the cell-cycle-control system in higher plants, we isolated, from an Arabidopsis thaliana cDNA library, two clones (CDC2a and CDC2b) similar to the Schizosacchawmyces pombe cdc2 gene. Genomic Southern-blot analysis with the CDC2a and CDC2b cDNA probes suggested that the A. thaliana genome contains several additional cdc2-like genes, which together with the CDC2a and CDC2b genes may constitute a CDC2 gene family. The CDC2a cDNA expressed in Sc. pombe corrected the elongated morphology, caused by the temperature-sensitive cdc2-33 mutation, to the normal shapes, indicating that the A. thaliana CDC2a gene product resembles Sc. pombe p34cdc2 functionally as well as structurally. These results support the view that the cell cycle of higher plants is controlled by an analogue of a p34cdc2-centered regulatory system like that of yeasts and animals.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalGene
Volume105
Issue number2
DOIs
Publication statusPublished - Sep 15 1991
Externally publishedYes

Fingerprint

Arabidopsis
Cell Cycle
Genes
Cell Cycle Checkpoints
Complementary DNA
Yeasts
Southern Blotting
Gene Library
Clone Cells
Genome
Mutation
Temperature

Keywords

  • cDNA
  • gene expression
  • higher plant
  • nucleotide sequence
  • Recombinant DNA
  • Schizosacchawmyces pombe

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana. / Hirayama, Takashi; Yoshiro, Imajuku; Toyoaki, Anai; Minami, Matsui; Atsuhiro, Oka.

In: Gene, Vol. 105, No. 2, 15.09.1991, p. 159-165.

Research output: Contribution to journalArticle

Hirayama, Takashi ; Yoshiro, Imajuku ; Toyoaki, Anai ; Minami, Matsui ; Atsuhiro, Oka. / Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana. In: Gene. 1991 ; Vol. 105, No. 2. pp. 159-165.
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