Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies

Hideki Matsui, S. Etoh, H. Nishio, M. Tokuda, T. Itano, O. Hatase

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Calcineurin (calcium- and calmodulin- stimulated phosphatase) α subunit purified from bovine brain was found to be composed of two polypeptides, 61 KDa (α1) and 59 KDa (α2). The two peptides were separated and extracted from polyacrylamide gel. The immuno-peptide mapping of the purified peptides by partial proteolysis showed that the 59-KDa polypeptide was not a degradative product of the 61-KDa polypeptide. The interaction of the enzyme with two monoclonal antibodies, Vj6 and Vd3, raised against bovine brain calcineurin revealed that the 61-KDa polypeptide was recognized by both Vj6 and Vd3, whereas the 59-KDa one was recognized only by Vj6. These results indicate that there are at least two isoforms of calcineurin a subunits in bovine brain.

Original languageEnglish
Pages (from-to)1119-1125
Number of pages7
JournalBiochemistry International
Volume24
Issue number6
Publication statusPublished - 1991
Externally publishedYes

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Calcineurin
Brain
Protein Isoforms
Monoclonal Antibodies
Peptides
Peptide Mapping
Proteolysis
Calmodulin
Phosphoric Monoester Hydrolases
Calcium
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Matsui, H., Etoh, S., Nishio, H., Tokuda, M., Itano, T., & Hatase, O. (1991). Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies. Biochemistry International, 24(6), 1119-1125.

Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies. / Matsui, Hideki; Etoh, S.; Nishio, H.; Tokuda, M.; Itano, T.; Hatase, O.

In: Biochemistry International, Vol. 24, No. 6, 1991, p. 1119-1125.

Research output: Contribution to journalArticle

Matsui, H, Etoh, S, Nishio, H, Tokuda, M, Itano, T & Hatase, O 1991, 'Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies', Biochemistry International, vol. 24, no. 6, pp. 1119-1125.
Matsui, Hideki ; Etoh, S. ; Nishio, H. ; Tokuda, M. ; Itano, T. ; Hatase, O. / Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies. In: Biochemistry International. 1991 ; Vol. 24, No. 6. pp. 1119-1125.
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