Abstract
Calcineurin (calcium- and calmodulin- stimulated phosphatase) α subunit purified from bovine brain was found to be composed of two polypeptides, 61 KDa (α1) and 59 KDa (α2). The two peptides were separated and extracted from polyacrylamide gel. The immuno-peptide mapping of the purified peptides by partial proteolysis showed that the 59-KDa polypeptide was not a degradative product of the 61-KDa polypeptide. The interaction of the enzyme with two monoclonal antibodies, Vj6 and Vd3, raised against bovine brain calcineurin revealed that the 61-KDa polypeptide was recognized by both Vj6 and Vd3, whereas the 59-KDa one was recognized only by Vj6. These results indicate that there are at least two isoforms of calcineurin a subunits in bovine brain.
| Original language | English |
|---|---|
| Pages (from-to) | 1119-1125 |
| Number of pages | 7 |
| Journal | Biochemistry International |
| Volume | 24 |
| Issue number | 6 |
| Publication status | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
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Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies. / Matsui, Hideki; Etoh, S.; Nishio, H.; Tokuda, M.; Itano, T.; Hatase, O.
In: Biochemistry International, Vol. 24, No. 6, 1991, p. 1119-1125.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of two calcineurin α isoforms in bovine brain by two different monoclonal antibodies
AU - Matsui, Hideki
AU - Etoh, S.
AU - Nishio, H.
AU - Tokuda, M.
AU - Itano, T.
AU - Hatase, O.
PY - 1991
Y1 - 1991
N2 - Calcineurin (calcium- and calmodulin- stimulated phosphatase) α subunit purified from bovine brain was found to be composed of two polypeptides, 61 KDa (α1) and 59 KDa (α2). The two peptides were separated and extracted from polyacrylamide gel. The immuno-peptide mapping of the purified peptides by partial proteolysis showed that the 59-KDa polypeptide was not a degradative product of the 61-KDa polypeptide. The interaction of the enzyme with two monoclonal antibodies, Vj6 and Vd3, raised against bovine brain calcineurin revealed that the 61-KDa polypeptide was recognized by both Vj6 and Vd3, whereas the 59-KDa one was recognized only by Vj6. These results indicate that there are at least two isoforms of calcineurin a subunits in bovine brain.
AB - Calcineurin (calcium- and calmodulin- stimulated phosphatase) α subunit purified from bovine brain was found to be composed of two polypeptides, 61 KDa (α1) and 59 KDa (α2). The two peptides were separated and extracted from polyacrylamide gel. The immuno-peptide mapping of the purified peptides by partial proteolysis showed that the 59-KDa polypeptide was not a degradative product of the 61-KDa polypeptide. The interaction of the enzyme with two monoclonal antibodies, Vj6 and Vd3, raised against bovine brain calcineurin revealed that the 61-KDa polypeptide was recognized by both Vj6 and Vd3, whereas the 59-KDa one was recognized only by Vj6. These results indicate that there are at least two isoforms of calcineurin a subunits in bovine brain.
UR - http://www.scopus.com/inward/record.url?scp=0026018333&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026018333&partnerID=8YFLogxK
M3 - Article
C2 - 1723595
AN - SCOPUS:0026018333
VL - 24
SP - 1119
EP - 1125
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 6
ER -