Identification of the site of iodide photooxidation in the photosystem II reaction center complex

Yuichiro Takahashi, Masa aki Takahashi, Kimiyuki Satoh

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    The highly purified PS II reaction center complex with 47, 43, 32, 30 and 9 kDa (and 4.5 kDa) polypeptides was subjected to iodo-labeling to identify the polypeptide(s) participating on the donor side of the photosystem. The 32 kDa component (D2) and the aggregate(s) of about 60 kDa were exclusively labeled in the dark, suggesting the presence of a dark-stable oxidizing component on this polypeptide. Illumination of the complex in the presence of 2,6-dichlorophenolindophenol and iodide resulted in the preferential iodination of the 30 kDa component (D1) and the aggregate(s), but not the 47 kDa polypeptide. This iodo-labeling was completely inhibited by the addition of an electron donor, 1,5-diphenylcarbazide. From these results, it is concluded that the site of photooxidation of iodide, and presumably the site of Z binding, is located on or near the herbicide-binding polypeptide, D1, in the PS II reaction center complex.

    Original languageEnglish
    Pages (from-to)347-351
    Number of pages5
    JournalFEBS Letters
    Volume208
    Issue number2
    DOIs
    Publication statusPublished - Nov 24 1986

    Keywords

    • Photosystem II Reaction center Iodo-labeling Secondary electron donor Herbicide-binding protein

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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