Identification of the site of iodide photooxidation in the photosystem II reaction center complex

Yuichiro Takahashi, Masa aki Takahashi, Kimiyuki Satoh

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The highly purified PS II reaction center complex with 47, 43, 32, 30 and 9 kDa (and 4.5 kDa) polypeptides was subjected to iodo-labeling to identify the polypeptide(s) participating on the donor side of the photosystem. The 32 kDa component (D2) and the aggregate(s) of about 60 kDa were exclusively labeled in the dark, suggesting the presence of a dark-stable oxidizing component on this polypeptide. Illumination of the complex in the presence of 2,6-dichlorophenolindophenol and iodide resulted in the preferential iodination of the 30 kDa component (D1) and the aggregate(s), but not the 47 kDa polypeptide. This iodo-labeling was completely inhibited by the addition of an electron donor, 1,5-diphenylcarbazide. From these results, it is concluded that the site of photooxidation of iodide, and presumably the site of Z binding, is located on or near the herbicide-binding polypeptide, D1, in the PS II reaction center complex.

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalFEBS Letters
Volume208
Issue number2
DOIs
Publication statusPublished - Nov 24 1986

Fingerprint

Photosystem II Protein Complex
Photooxidation
Iodides
Peptides
Labeling
Diphenylcarbazide
2,6-Dichloroindophenol
Halogenation
Herbicides
Lighting
Binding Sites
Electrons

Keywords

  • Photosystem II Reaction center Iodo-labeling Secondary electron donor Herbicide-binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of the site of iodide photooxidation in the photosystem II reaction center complex. / Takahashi, Yuichiro; Takahashi, Masa aki; Satoh, Kimiyuki.

In: FEBS Letters, Vol. 208, No. 2, 24.11.1986, p. 347-351.

Research output: Contribution to journalArticle

Takahashi, Yuichiro ; Takahashi, Masa aki ; Satoh, Kimiyuki. / Identification of the site of iodide photooxidation in the photosystem II reaction center complex. In: FEBS Letters. 1986 ; Vol. 208, No. 2. pp. 347-351.
@article{abb13ba428634b72a51100447c842640,
title = "Identification of the site of iodide photooxidation in the photosystem II reaction center complex",
abstract = "The highly purified PS II reaction center complex with 47, 43, 32, 30 and 9 kDa (and 4.5 kDa) polypeptides was subjected to iodo-labeling to identify the polypeptide(s) participating on the donor side of the photosystem. The 32 kDa component (D2) and the aggregate(s) of about 60 kDa were exclusively labeled in the dark, suggesting the presence of a dark-stable oxidizing component on this polypeptide. Illumination of the complex in the presence of 2,6-dichlorophenolindophenol and iodide resulted in the preferential iodination of the 30 kDa component (D1) and the aggregate(s), but not the 47 kDa polypeptide. This iodo-labeling was completely inhibited by the addition of an electron donor, 1,5-diphenylcarbazide. From these results, it is concluded that the site of photooxidation of iodide, and presumably the site of Z binding, is located on or near the herbicide-binding polypeptide, D1, in the PS II reaction center complex.",
keywords = "Photosystem II Reaction center Iodo-labeling Secondary electron donor Herbicide-binding protein",
author = "Yuichiro Takahashi and Takahashi, {Masa aki} and Kimiyuki Satoh",
year = "1986",
month = "11",
day = "24",
doi = "10.1016/0014-5793(86)81047-X",
language = "English",
volume = "208",
pages = "347--351",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Identification of the site of iodide photooxidation in the photosystem II reaction center complex

AU - Takahashi, Yuichiro

AU - Takahashi, Masa aki

AU - Satoh, Kimiyuki

PY - 1986/11/24

Y1 - 1986/11/24

N2 - The highly purified PS II reaction center complex with 47, 43, 32, 30 and 9 kDa (and 4.5 kDa) polypeptides was subjected to iodo-labeling to identify the polypeptide(s) participating on the donor side of the photosystem. The 32 kDa component (D2) and the aggregate(s) of about 60 kDa were exclusively labeled in the dark, suggesting the presence of a dark-stable oxidizing component on this polypeptide. Illumination of the complex in the presence of 2,6-dichlorophenolindophenol and iodide resulted in the preferential iodination of the 30 kDa component (D1) and the aggregate(s), but not the 47 kDa polypeptide. This iodo-labeling was completely inhibited by the addition of an electron donor, 1,5-diphenylcarbazide. From these results, it is concluded that the site of photooxidation of iodide, and presumably the site of Z binding, is located on or near the herbicide-binding polypeptide, D1, in the PS II reaction center complex.

AB - The highly purified PS II reaction center complex with 47, 43, 32, 30 and 9 kDa (and 4.5 kDa) polypeptides was subjected to iodo-labeling to identify the polypeptide(s) participating on the donor side of the photosystem. The 32 kDa component (D2) and the aggregate(s) of about 60 kDa were exclusively labeled in the dark, suggesting the presence of a dark-stable oxidizing component on this polypeptide. Illumination of the complex in the presence of 2,6-dichlorophenolindophenol and iodide resulted in the preferential iodination of the 30 kDa component (D1) and the aggregate(s), but not the 47 kDa polypeptide. This iodo-labeling was completely inhibited by the addition of an electron donor, 1,5-diphenylcarbazide. From these results, it is concluded that the site of photooxidation of iodide, and presumably the site of Z binding, is located on or near the herbicide-binding polypeptide, D1, in the PS II reaction center complex.

KW - Photosystem II Reaction center Iodo-labeling Secondary electron donor Herbicide-binding protein

UR - http://www.scopus.com/inward/record.url?scp=0003198870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0003198870&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(86)81047-X

DO - 10.1016/0014-5793(86)81047-X

M3 - Article

AN - SCOPUS:0003198870

VL - 208

SP - 347

EP - 351

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -