Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus)

Kei Tamura, Yasuhisa Kobayashi, Asuka Hirooka, Keiko Takanami, Takumi Oti, Takamichi Jogahara, Sen Ichi Oda, Tatsuya Sakamoto, Hirotaka Sakamoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Several regions of the brain and spinal cord control male reproductive function. We previously demonstrated that the gastrin-releasing peptide (GRP) system, located in the lumbosacral spinal cord of rats, controls spinal centers to promote penile reflexes during male copulatory behavior. However, little information exists on the male-specific spinal GRP system in animals other than rats. The objective of this study was to examine the functional generality of the spinal GRP system in mammals using the Asian house musk shrew (Suncus murinus; suncus named as the laboratory strain), a specialized placental mammal model. Mice are also used for a representative model of small laboratory animals. We first isolated complementary DNA encoding GRP in suncus. Phylogenetic analysis revealed that suncus preproGRP was clustered to an independent branch. Reverse transcription-PCR showed that GRP and its receptor mRNAs were both expressed in the lumbar spinal cord of suncus and mice. Immunohistochemistry for GRP demonstrated that the sexually dimorphic GRP system and male-specific expression/distribution patterns of GRP in the lumbosacral spinal cord in suncus are similar to those of mice. In suncus, we further found that most GRP-expressing neurons in males also express androgen receptors, suggesting that this male-dominant system in suncus is also androgen-dependent. Taken together, these results indicate that the sexually dimorphic spinal GRP system exists not only in mice but also in suncus, suggesting that this system is a conserved property in mammals. J. Comp. Neurol. 525:1586–1598, 2017.

Original languageEnglish
Pages (from-to)1586-1598
Number of pages13
JournalJournal of Comparative Neurology
Volume525
Issue number7
DOIs
Publication statusPublished - May 1 2017

Fingerprint

Gastrin-Releasing Peptide
Shrews
Spinal Cord
Mammals
Bombesin Receptors
musk
Androgen Receptors
Androgens
Reverse Transcription
Reflex
Animal Models
Complementary DNA
Immunohistochemistry
Neurons

Keywords

  • gastrin-releasing peptide
  • male reproductive function
  • RRID: AB_2060157
  • RRID: AB_2571636
  • RRID: AB_626757
  • sexual dimorphism
  • spinal cord
  • Suncus murinus (suncus)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus). / Tamura, Kei; Kobayashi, Yasuhisa; Hirooka, Asuka; Takanami, Keiko; Oti, Takumi; Jogahara, Takamichi; Oda, Sen Ichi; Sakamoto, Tatsuya; Sakamoto, Hirotaka.

In: Journal of Comparative Neurology, Vol. 525, No. 7, 01.05.2017, p. 1586-1598.

Research output: Contribution to journalArticle

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