Identification of the PEG-induced proteins by 2D-gel electrophoresis and mass spectrometry in Sphingopyxis macrogoltabida strain 103

Jittima Charoenpanich, Akio Tani, Fusako Kawai

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Cell-free extracts from a PEG 4000-utilizing bacterium, Sphingopyxis macrogoltabida strain 103, grown on glucose and PEG 4000 medium were separated into cytoplasmic, membrane-bound and signal protein fractions. Each fraction was analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). A total of 19 differentially-expressed proteins by PEG were in-gel trypsin digested and the digestion mixtures were analyzed by MALDI-TOF mass spectrometry to determine the molecular masses of the resulting tryptic peptides. Ten proteins in the cytoplasmic fraction showed homology to fatty acyl CoA synthetase, IEA two-component response regulator, permease, LacI-transcription regulator, galactinol synthase, coenzyme PQQ synthesis protein, transcription regulator, translation-initiation factor like protein, CheY-like two-component response regulator and hypothetical protein. Three proteins in the membrane-bound fraction were identified as LysR-transcription regulator, GutR-transcription regulator and two-component response regulator. Six proteins in the signal protein fraction were polyphosphate kinase, ATP sulfurylase, amino acid permease, sigma-54 dependent transcription regulator, fatty-acid-CoA ligase and LysR-transcription regulator. These proteins are expected to be relevant to PEG metabolism by S. macrogoltabida strain 103.

    Original languageEnglish
    Pages (from-to)111-124
    Number of pages14
    JournalChiang Mai University Journal of Natural Sciences
    Volume9
    Issue number1
    Publication statusPublished - Jan 1 2010

    Keywords

    • 2D-PAGE
    • MALDI-TOF mass spectrometry
    • PEG degradation
    • Sphingopyxis macrogoltabida

    ASJC Scopus subject areas

    • General

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