Identification of the PEG-induced proteins by 2D-gel electrophoresis and mass spectrometry in Sphingopyxis macrogoltabida strain 103

Jittima Charoenpanich, Akiwo Tani, Fusako Kawai

Research output: Contribution to journalArticle

Abstract

Cell-free extracts from a PEG 4000-utilizing bacterium, Sphingopyxis macrogoltabida strain 103, grown on glucose and PEG 4000 medium were separated into cytoplasmic, membrane-bound and signal protein fractions. Each fraction was analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). A total of 19 differentially-expressed proteins by PEG were in-gel trypsin digested and the digestion mixtures were analyzed by MALDI-TOF mass spectrometry to determine the molecular masses of the resulting tryptic peptides. Ten proteins in the cytoplasmic fraction showed homology to fatty acyl CoA synthetase, IEA two-component response regulator, permease, LacI-transcription regulator, galactinol synthase, coenzyme PQQ synthesis protein, transcription regulator, translation-initiation factor like protein, CheY-like two-component response regulator and hypothetical protein. Three proteins in the membrane-bound fraction were identified as LysR-transcription regulator, GutR-transcription regulator and two-component response regulator. Six proteins in the signal protein fraction were polyphosphate kinase, ATP sulfurylase, amino acid permease, sigma-54 dependent transcription regulator, fatty-acid-CoA ligase and LysR-transcription regulator. These proteins are expected to be relevant to PEG metabolism by S. macrogoltabida strain 103.

Original languageEnglish
Pages (from-to)111-124
Number of pages14
JournalChiang Mai University Journal of Natural Sciences
Volume9
Issue number1
Publication statusPublished - Jan 2010

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Sphingomonas macrogoltabidus
gel electrophoresis
mass spectrometry
transcription factors
proteins
polyacrylamide gel electrophoresis
inositol 3-alpha-galactosyltransferase
sulfate adenylyltransferase
long-chain-fatty-acid-CoA ligase
polyphosphates
coenzymes
matrix-assisted laser desorption-ionization mass spectrometry
two-dimensional gel electrophoresis
ligases
membrane proteins
trypsin
translation (genetics)
cell membranes
phosphotransferases (kinases)
protein synthesis

Keywords

  • 2D-PAGE
  • MALDI-TOF mass spectrometry
  • PEG degradation
  • Sphingopyxis macrogoltabida

ASJC Scopus subject areas

  • General

Cite this

Identification of the PEG-induced proteins by 2D-gel electrophoresis and mass spectrometry in Sphingopyxis macrogoltabida strain 103. / Charoenpanich, Jittima; Tani, Akiwo; Kawai, Fusako.

In: Chiang Mai University Journal of Natural Sciences, Vol. 9, No. 1, 01.2010, p. 111-124.

Research output: Contribution to journalArticle

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