Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii

Hiroko Takahashi, Masakazu Iwai, Yuichiro Takahashi, Jun Minagawa

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

State transition in photosynthesis is a short-term balancing mechanism of energy distribution between photosystem I (PSI) and photosystem II (PSII). When PSII is preferentially excited (state 2), a pool of mobile light-harvesting complex II (LHCII) antenna proteins is thought to migrate from PSII to PSI, but biochemical evidence for a physical association between LHCII proteins and PSI in state 2 is weak. Here, using the green alga Chlamydomonas reinhardtii, which has a high capacity for state transitions, we report the isolation of PSI-light-harvesting complex I (LHCI) super-complexes from cells locked into state 1 and state 2. We solubilized the thylakoid membranes with a mild detergent, separated the proteins by sucrose density gradient centrifugation, and subjected gradient fractions to gel-filtration chromatography. Three LHCII polypeptides were associated with a PSI-LHCI supercomplex only in state 2; we identified them as two minor monomeric LHCII proteins (CP26 and CP29) and one previously unreported major LHCII protein type II, or LhcbM5. These three LHCII proteins, in addition to the major trimeric LHCII proteins, were phosphorylated upon transition to state 2. The corresponding phylogenetic tree indicates that among the LHCII proteins associated with PSII, these three LHCII proteins are the most similar to the LHC proteins for PSI, (LHCI). Our results are important because CP26, CP29, and LhcbM5, which have been viewed as belonging solely to the PSII complex, are now postulated to shuttle between PSI and PSII during state transitions, thereby acting as docking sites for the trimeric LHCII proteins in both PSI and PSII.

Original languageEnglish
Pages (from-to)477-482
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number2
DOIs
Publication statusPublished - Jan 10 2006

Fingerprint

Light-Harvesting Protein Complexes
Chlamydomonas reinhardtii
Photosystem I Protein Complex
Photosystem II Protein Complex
Light
Peptides
Chlorophyta
Thylakoids
Proteins
Density Gradient Centrifugation
Photosynthesis
Detergents
Gel Chromatography
Sucrose

Keywords

  • Photoacclimation
  • Photosynthesis
  • Photosystem

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. / Takahashi, Hiroko; Iwai, Masakazu; Takahashi, Yuichiro; Minagawa, Jun.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 2, 10.01.2006, p. 477-482.

Research output: Contribution to journalArticle

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