Identification of the centromere-specific histone H3 variant in Lotus japonicus

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The centromere is a structurally and functionally specialized region present on every eukaryotic chromosome. Lotus japonicus is a model legume species for which there is very limited information on the centromere structure. Here we cloned and characterized the L. japonicus homolog of the centromere-specific histone H3 gene (LjCenH3) encoding a 159-amino acid protein. Using an Agrobacterium-based transformation system, LjCenH3 tagged with a green fluorescent protein was transferred into L. japonicus cells. The centromeric position of LjCENH3 protein was revealed on L. japonicus metaphase chromosomes by an immunofluorescence assay. The identification of LjCenH3 as a critical centromere landmark could pave the way for a better understanding of centromere structure in this model and other agriculturally important legume species.

Original languageEnglish
Pages (from-to)8-11
Number of pages4
JournalGene
Volume538
Issue number1
DOIs
Publication statusPublished - Mar 15 2014

Fingerprint

Centromere
Histones
Fabaceae
Chromosomes
Agrobacterium
Metaphase
Green Fluorescent Proteins
Fluorescent Antibody Technique
Proteins
Loteae
Amino Acids
Genes

Keywords

  • CenH3/CENP-A
  • Centromere
  • Fabaceae
  • Green fluorescent protein
  • Leguminosae

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of the centromere-specific histone H3 variant in Lotus japonicus. / Tek, Ahmet L.; Kashihara, Kazunari; Murata, Minoru; Nagaki, Kiyotaka.

In: Gene, Vol. 538, No. 1, 15.03.2014, p. 8-11.

Research output: Contribution to journalArticle

Tek, Ahmet L. ; Kashihara, Kazunari ; Murata, Minoru ; Nagaki, Kiyotaka. / Identification of the centromere-specific histone H3 variant in Lotus japonicus. In: Gene. 2014 ; Vol. 538, No. 1. pp. 8-11.
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