Identification of the centromere-specific histone H3 variant in Lotus japonicus

Ahmet L. Tek; Kazunari Kashihara; Minoru Murata; Kiyotaka Nagaki

doi:10.1016/j.gene.2014.01.034

Identification of the centromere-specific histone H3 variant in Lotus japonicus

Ahmet L. Tek, Kazunari Kashihara, Minoru Murata, Kiyotaka Nagaki

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The centromere is a structurally and functionally specialized region present on every eukaryotic chromosome. Lotus japonicus is a model legume species for which there is very limited information on the centromere structure. Here we cloned and characterized the L. japonicus homolog of the centromere-specific histone H3 gene (LjCenH3) encoding a 159-amino acid protein. Using an Agrobacterium-based transformation system, LjCenH3 tagged with a green fluorescent protein was transferred into L. japonicus cells. The centromeric position of LjCENH3 protein was revealed on L. japonicus metaphase chromosomes by an immunofluorescence assay. The identification of LjCenH3 as a critical centromere landmark could pave the way for a better understanding of centromere structure in this model and other agriculturally important legume species.

Original language	English
Pages (from-to)	8-11
Number of pages	4
Journal	Gene
Volume	538
Issue number	1
DOIs	https://doi.org/10.1016/j.gene.2014.01.034
Publication status	Published - Mar 15 2014

Keywords

CenH3/CENP-A
Centromere
Fabaceae
Green fluorescent protein
Leguminosae

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2014.01.034

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title = "Identification of the centromere-specific histone H3 variant in Lotus japonicus",

abstract = "The centromere is a structurally and functionally specialized region present on every eukaryotic chromosome. Lotus japonicus is a model legume species for which there is very limited information on the centromere structure. Here we cloned and characterized the L. japonicus homolog of the centromere-specific histone H3 gene (LjCenH3) encoding a 159-amino acid protein. Using an Agrobacterium-based transformation system, LjCenH3 tagged with a green fluorescent protein was transferred into L. japonicus cells. The centromeric position of LjCENH3 protein was revealed on L. japonicus metaphase chromosomes by an immunofluorescence assay. The identification of LjCenH3 as a critical centromere landmark could pave the way for a better understanding of centromere structure in this model and other agriculturally important legume species.",

keywords = "CenH3/CENP-A, Centromere, Fabaceae, Green fluorescent protein, Leguminosae",

author = "Tek, {Ahmet L.} and Kazunari Kashihara and Minoru Murata and Kiyotaka Nagaki",

note = "Funding Information: We thank to Dr. Douglas R. Cook of University of California, Davis, USA, Dr. Ingo Schubert of Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany and anonymous reviewers for valuable contributions and critical reading of the manuscript. This work was supported by the Fellowship Programs of the Japan Society for the Promotion of Science (JSPS), the Turkish Higher Education Council, and Harran University.",

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AU - Tek, Ahmet L.

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AU - Murata, Minoru

AU - Nagaki, Kiyotaka

N1 - Funding Information: We thank to Dr. Douglas R. Cook of University of California, Davis, USA, Dr. Ingo Schubert of Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany and anonymous reviewers for valuable contributions and critical reading of the manuscript. This work was supported by the Fellowship Programs of the Japan Society for the Promotion of Science (JSPS), the Turkish Higher Education Council, and Harran University.

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N2 - The centromere is a structurally and functionally specialized region present on every eukaryotic chromosome. Lotus japonicus is a model legume species for which there is very limited information on the centromere structure. Here we cloned and characterized the L. japonicus homolog of the centromere-specific histone H3 gene (LjCenH3) encoding a 159-amino acid protein. Using an Agrobacterium-based transformation system, LjCenH3 tagged with a green fluorescent protein was transferred into L. japonicus cells. The centromeric position of LjCENH3 protein was revealed on L. japonicus metaphase chromosomes by an immunofluorescence assay. The identification of LjCenH3 as a critical centromere landmark could pave the way for a better understanding of centromere structure in this model and other agriculturally important legume species.

AB - The centromere is a structurally and functionally specialized region present on every eukaryotic chromosome. Lotus japonicus is a model legume species for which there is very limited information on the centromere structure. Here we cloned and characterized the L. japonicus homolog of the centromere-specific histone H3 gene (LjCenH3) encoding a 159-amino acid protein. Using an Agrobacterium-based transformation system, LjCenH3 tagged with a green fluorescent protein was transferred into L. japonicus cells. The centromeric position of LjCENH3 protein was revealed on L. japonicus metaphase chromosomes by an immunofluorescence assay. The identification of LjCenH3 as a critical centromere landmark could pave the way for a better understanding of centromere structure in this model and other agriculturally important legume species.

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KW - Green fluorescent protein

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Identification of the centromere-specific histone H3 variant in Lotus japonicus

Abstract

Keywords

ASJC Scopus subject areas

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