Identification of the carboxyl-terminal processing protease for the D1 precursor protein of the photosystem II reaction center of spinach

Shuji Fujita, Noritoshi Inagaki, Yumiko Yamamoto, Fumiko Taguchi, Akira Matsumoto, Kimiyuki Satoh

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The enzyme involved in the carboxyl-terminal processing of the D1 precursor protein (pD1) of the photosystem II reaction center was purified from extracts of sonicated spinach thylakoids by a method that included chromatography on quaternary aminoethyl anion-exchange, hydroxylapatite, copper-chelating affinity and gel-filtration columns. The enzyme was identified, from its chromatographic behavior, to be a monomeric protein of about 45 kDa. The sequence of the amino-terminal 27 amino acids of this protein was determined directly, which exhibited low but appreciable (37%) homology to that deduced from a gene (ctpA) in Synechocystis sp. PCC 6803 that was proposed recently to encode the processing protease from results of genetic complementation analysis.

Original languageEnglish
Pages (from-to)1169-1177
Number of pages9
JournalPlant and Cell Physiology
Volume36
Issue number7
Publication statusPublished - Oct 1995

Fingerprint

Reaction Center
Photosystem II
Spinach
Photosystem II Protein Complex
Protein Precursors
Spinacia oleracea
Protease
spinach
Precursor
photosystem II
Peptide Hydrolases
proteinases
Proteins
Protein
Synechocystis
Thylakoids
protein
Enzymes
Durapatite
Processing

Keywords

  • C-terminal processing
  • CtpA
  • D1 protein
  • Photosystem II
  • Protease
  • Spinach (Spinacia oleracea)

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Identification of the carboxyl-terminal processing protease for the D1 precursor protein of the photosystem II reaction center of spinach. / Fujita, Shuji; Inagaki, Noritoshi; Yamamoto, Yumiko; Taguchi, Fumiko; Matsumoto, Akira; Satoh, Kimiyuki.

In: Plant and Cell Physiology, Vol. 36, No. 7, 10.1995, p. 1169-1177.

Research output: Contribution to journalArticle

Fujita, Shuji ; Inagaki, Noritoshi ; Yamamoto, Yumiko ; Taguchi, Fumiko ; Matsumoto, Akira ; Satoh, Kimiyuki. / Identification of the carboxyl-terminal processing protease for the D1 precursor protein of the photosystem II reaction center of spinach. In: Plant and Cell Physiology. 1995 ; Vol. 36, No. 7. pp. 1169-1177.
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