Identification of the carboxyl-terminal processing protease for the D1 precursor protein of the photosystem II reaction center of spinach

Shuji Fujita, Noritoshi Inagaki, Yumiko Yamamoto, Fumiko Taguchi, Akira Matsumoto, Kimiyuki Satoh

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The enzyme involved in the carboxyl-terminal processing of the D1 precursor protein (pD1) of the photosystem II reaction center was purified from extracts of sonicated spinach thylakoids by a method that included chromatography on quaternary aminoethyl anion-exchange, hydroxylapatite, copper-chelating affinity and gel-filtration columns. The enzyme was identified, from its chromatographic behavior, to be a monomeric protein of about 45 kDa. The sequence of the amino-terminal 27 amino acids of this protein was determined directly, which exhibited low but appreciable (37%) homology to that deduced from a gene (ctpA) in Synechocystis sp. PCC 6803 that was proposed recently to encode the processing protease from results of genetic complementation analysis.

Original languageEnglish
Pages (from-to)1169-1177
Number of pages9
JournalPlant and Cell Physiology
Volume36
Issue number7
Publication statusPublished - Oct 1 1995

Keywords

  • C-terminal processing
  • CtpA
  • D1 protein
  • Photosystem II
  • Protease
  • Spinach (Spinacia oleracea)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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