Identification of testis specific calcineurin β subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence

Hajime Nishio, Hideki Matsui, Seiji Etoh, Lizomar J M P Moia, Masaaki Tokuda, Toshifumi Itano, Osamu Hatase

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two isoforms of calcineurin β subunit(β1 and β2) were identified in rat testis by a monoclonal antibody Val. Both β1 and β2 were recovered in calmodulin binding protein fraction and showed calcium shift on SDS-polyacrylamide gel electrophoresis which is the specific character for EF-hand calcium binding protein. β2 showed same apparent molecular weight on SDS-PAGE as that of brain calcineurin and was found in wide variety of tissues. β1 was shown to have six amino acid polypepeptide sequence and it showed higher molecular weight than brain β and was specific for testis.

Original languageEnglish
Pages (from-to)34-38
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number1
DOIs
Publication statusPublished - Jan 15 1992
Externally publishedYes

Fingerprint

Calcineurin
Testis
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Brain
Protein Isoforms
Molecular Weight
Molecular weight
Monoclonal Antibodies
Calmodulin-Binding Proteins
EF Hand Motifs
Amino Acids
Calcium-Binding Proteins
Electrophoresis
Rats
Tissue
Calcium
polyacrylamide gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of testis specific calcineurin β subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence. / Nishio, Hajime; Matsui, Hideki; Etoh, Seiji; Moia, Lizomar J M P; Tokuda, Masaaki; Itano, Toshifumi; Hatase, Osamu.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 1, 15.01.1992, p. 34-38.

Research output: Contribution to journalArticle

Nishio, Hajime ; Matsui, Hideki ; Etoh, Seiji ; Moia, Lizomar J M P ; Tokuda, Masaaki ; Itano, Toshifumi ; Hatase, Osamu. / Identification of testis specific calcineurin β subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 182, No. 1. pp. 34-38.
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