Identification of testis specific calcineurin β subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence

Hajime Nishio, Hideki Matsui, Seiji Etoh, Lizomar J.M.P. Moia, Masaaki Tokuda, Toshifumi Itano, Osamu Hatase

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two isoforms of calcineurin β subunit(β1 and β2) were identified in rat testis by a monoclonal antibody Val. Both β1 and β2 were recovered in calmodulin binding protein fraction and showed calcium shift on SDS-polyacrylamide gel electrophoresis which is the specific character for EF-hand calcium binding protein. β2 showed same apparent molecular weight on SDS-PAGE as that of brain calcineurin and was found in wide variety of tissues. β1 was shown to have six amino acid polypepeptide sequence and it showed higher molecular weight than brain β and was specific for testis.

Original languageEnglish
Pages (from-to)34-38
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number1
DOIs
Publication statusPublished - Jan 15 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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