Identification of STOP1-like proteins associated with aluminum tolerance in sweet sorghum (Sorghum bicolor l.)

Sheng Huang, Jie Gao, Jiangfeng You, Yanan Liang, Kexing Guan, Siqi Yan, Meiqi Zhan, Zhenming Yang

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Aluminum (Al) toxicity in acidic soils affects crop production worldwide. C2 H2 -type zinc finger transcription factor STOP1/ART1-mediated expression of Al tolerance genes has been shown to be important for Al resistance in Arabidopsis, rice and other crop plants. Here, we identified and characterized four STOP1-like proteins (SbSTOP1a, SbSTOP1b, SbSTOP1c, and SbSTOP1d) in sweet sorghum, a variant of grain sorghum (Sorghum bicolor L.). Al induced the transcription of the four SbSTOP1 genes in both time- and Al concentration-dependent manners. All SbSTOP1 proteins localized to the cell nucleus, and they showed transcriptional activity in a yeast expression system. In the HEK 293 coexpression system, SbSTOP1d showed transcriptional regulation of SbSTAR2 and SbMATE, indicating the possible existence of another SbSTOP1 and SbSTAR2-dependent Al tolerance mechanism in sorghum apart from the reported SbMATE-mediated Al exclusion mechanism. A transgenic complementation assay showed that SbSTOP1d significantly rescued the Al-sensitivity characteristic of the Atstop1 mutant. Additionally, yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays showed that SbSTOP1d interacted with SbSTOP1b and SbSTOP1d itself, suggesting that SbSTOP1 may function as a homodimer and/or heterodimer. These results indicate that STOP1 plays an important role in Al tolerance in sweet sorghum and extend our understanding of the complex regulatory mechanisms of STOP1-like proteins in response to Al toxicity.

Original languageEnglish
Article number258
JournalFrontiers in Plant Science
Volume9
DOIs
Publication statusPublished - Feb 28 2018
Externally publishedYes

Keywords

  • Al tolerance genes
  • Aluminum toxicity
  • STOP1
  • Sweet sorghum
  • Transcriptional regulation

ASJC Scopus subject areas

  • Plant Science

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