Identification of specific autoantigens in Sjögren's syndrome by SEREX

Kazuo Uchida, Yoichi Akita, Keitaro Matsuo, Shigeyoshi Fujiwara, Atsuko Nakagawa, Yoshiaki Kazaoka, Hiroshi Hachiya, Yoshiyuki Naganawa, Ichiro Oh-Iwa, Kiyoshi Ohura, Shinsuke Saga, Tatsushi Kawai, Yoshinari Matsumoto, Kazuo Shimozato, Ken Ichi Kozaki

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We carried out SEREX (serological analysis of antigens by recombinant cDNA expression cloning) using sera from patients with Sjögren's syndrome (SjS) and investigated the frequencies of autoantibodies against autoantigens identified by SEREX in the sera of healthy individuals (HI) and patients with SjS, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). IFI16 and two kelch-like proteins, KLHL12 and KLHL7, were found to be novel autoantigens in SjS by SEREX. A markedly high frequency of anti-IFI16 autoantibodies was observed in the sera of SjS (SjS, 70%; RA, 13%; SLE, 33%; HI, 0%). Interestingly, all serum samples from SjS demonstrated immunoreactivity against one or both of IFI16 and SS-B/La. The presence of autoantibodies against KLHL12 and KLHL7 in the sera was significantly specific to SjS (23% and 17%, respectively), as they were not detected in RA, SLE or HI. Furthermore, we confirmed that transcripts of these autoantigens were expressed preferentially in the salivary glands and immuno-privileged testes. Our results suggest these autoantigens may be useful as serological markers for the clinical diagnosis of SjS and may play a crucial role as organ-specific autoantigens in the aetiopathogenesis of SjS. This study warranted clinical evaluations of autoantibodies against IFI16, KLHL12 and KLHL7 in combination with anti-SS-B/La autoantibodies.

Original languageEnglish
Pages (from-to)53-63
Number of pages11
JournalImmunology
Volume116
Issue number1
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Keywords

  • IFI16
  • KLHL12
  • KLHL7
  • SEREX
  • Sjogren's syndrome

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Identification of specific autoantigens in Sjögren's syndrome by SEREX'. Together they form a unique fingerprint.

  • Cite this

    Uchida, K., Akita, Y., Matsuo, K., Fujiwara, S., Nakagawa, A., Kazaoka, Y., Hachiya, H., Naganawa, Y., Oh-Iwa, I., Ohura, K., Saga, S., Kawai, T., Matsumoto, Y., Shimozato, K., & Kozaki, K. I. (2005). Identification of specific autoantigens in Sjögren's syndrome by SEREX. Immunology, 116(1), 53-63. https://doi.org/10.1111/j.1365-2567.2005.02197.x