Identification of RAPD markers linked to the Tm-2 locus in tomato

T. Ohmori, M. Murata, F. Motoyoshi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Tm-2 and Tm-2a are genes conferring resistance to tomato mosaic virus in Lycopersicon esculentum. They are allelic and originated from different lines of L. peruvianum, a wild relative of tomato. In this study, random amplified polymorphic DNA (RAPD) markers linked to these genes were screened in nearly isogenic lines (NILs). To detect RAPDs differentiating NILs, 220 different 10-base oligonucleotide primers were examined by the polymerase chain reaction (PCR), and 43 of them generated 53 consistent polymorphic fragments among the NILs. Out of these 53 fragments, 13 were arbitrarily chosen and examined in respect of whether they were linked to the netted virescent (nv) gene, since nv is tightly linked to the Tm-2 locus and its phenotype is more easily distinguishable. As a result, all 13 markers were shown to be linked to nv, and hence to the Tm-2 locus. Among them, two fragments specific to the NIL carrying Tm-2 three specific to the NIL carrying Tm-2a, and four specific to both of these NILs were closely linked to nv.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalTheoretical and Applied Genetics
Volume90
Issue number3-4
DOIs
Publication statusPublished - Mar 1 1995

Keywords

  • Disease resistance
  • Nearly isogenic lines (NILs)
  • Polymerase chain reaction (PCR)
  • Random amplified polymorphic DNA (RAPD)
  • Tomato mosaic virus

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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