Identification of qtls for rice grain size using a novel set of chromosomal segment substitution lines derived from yamadanishiki in the genetic background of koshihikari

Satoshi Okada, Akio Onogi, Ken Iijima, Kiyosumi Hori, Hiroyoshi Iwata, Wakana Yokoyama, Miki Suehiro, Masanori Yamasaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Grain size is important for brewing-rice cultivars, but the genetic basis for this trait is still unclear. This paper aims to identify QTLs for grain size using novel chromosomal segment substitution lines (CSSLs) harboring chromosomal segments from Yamadanishiki, an excellent sake-brewing rice, in the genetic background of Koshihikari, a cooking cultivar. We developed a set of 49 CSSLs. Grain length (GL), grain width (GWh), grain thickness (GT), 100-grain weight (GWt) and days to heading (DTH) were evaluated, and a CSSL-QTL analysis was conducted. Eighteen QTLs for grain size and DTH were identified. Seven (qGL11, qGWh5, qGWh10, qGWt6-2, qGWt10-2, qDTH3, and qDTH6) that were detected in F2 and recombinant inbred lines (RILs) from Koshihikari/Yamadanishiki were validated, suggesting that they are important for large grain size and heading date in Yamadanishiki. Additionally, QTL reanalysis for GWt showed that qGWt10-2 was only detected in early-flowering RILs, while qGWt5 (in the same region as qGWh5) was only detected in late-flowering RILs, suggesting that these QTLs show different responses to the environment. Our study revealed that grain size in the Yamadanishiki cultivar is determined by a complex genetic mechanism. These findings could be useful for the breeding of both cooking and brewing rice.

Original languageEnglish
Pages (from-to)210-218
Number of pages9
JournalBreeding Science
Volume68
Issue number2
DOIs
Publication statusPublished - Jan 1 2018
Externally publishedYes

Fingerprint

substitution lines
genetic background
Cooking
rice
Weights and Measures
quantitative trait loci
brewing
heading
Breeding
inbred lines
cooking
cultivars
Genetic Background
Oryza
flowering
sake

Keywords

  • Brewing-rice cultivar
  • CSSLs
  • Grain size
  • QTL
  • QTL-by-environment interaction

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Identification of qtls for rice grain size using a novel set of chromosomal segment substitution lines derived from yamadanishiki in the genetic background of koshihikari. / Okada, Satoshi; Onogi, Akio; Iijima, Ken; Hori, Kiyosumi; Iwata, Hiroyoshi; Yokoyama, Wakana; Suehiro, Miki; Yamasaki, Masanori.

In: Breeding Science, Vol. 68, No. 2, 01.01.2018, p. 210-218.

Research output: Contribution to journalArticle

Okada, Satoshi ; Onogi, Akio ; Iijima, Ken ; Hori, Kiyosumi ; Iwata, Hiroyoshi ; Yokoyama, Wakana ; Suehiro, Miki ; Yamasaki, Masanori. / Identification of qtls for rice grain size using a novel set of chromosomal segment substitution lines derived from yamadanishiki in the genetic background of koshihikari. In: Breeding Science. 2018 ; Vol. 68, No. 2. pp. 210-218.
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