Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice

Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, Takao Kinjo, Naoya Kobayashi, Shinji Kitamura, Issei Saitoh, Masami Watanabe, Jiro Fujita, Hirofumi Noguchi

Research output: Contribution to journalArticle

Abstract

Although cell therapy using adipose-derived mesenchymal stem cells (AdMSCs) regulates immunity, the degree to which cell quality and function are affected by differences in immunodeficiency of donors is unknown. We used liquid chromatography tandem-mass spectrometry (LC MS/MS) to identify the proteins expressed by mouse AdMSCs (mAsMSCs) isolated from normal (C57BL/6) mice and mice with severe combined immunodeficiency (SCID). The protein expression profiles of each strain were 98%-100% identical, indicating that the expression levels of major proteins potentially associated with the therapeutic effects of mAdMSCs were highly similar. Further, comparable levels of cell surface markers (CD44, CD90.2) were detected using flow cytometry or LC MS/MS. MYH9, ACTN1, CANX, GPI, TPM1, EPRS, ITGB1, ANXA3, CNN2, MAPK1, PSME2, CTPS1, OTUB1, PSMB6, HMGB1, RPS19, SEC61A1, CTNNB1, GLO1, RPL22, PSMA2, SYNCRIP, PRDX3, SAMHD1, TCAF2, MAPK3, RPS24, and MYO1E, which are associated with immunity, were expressed at higher levels by the SCID mAdMSCs compared with the C57BL/6 mAdMSCs. In contrast, ANXA9, PCBP2, LGALS3, PPP1R14B, and PSMA6, which are also associated with immunity, were more highly expressed by C57BL/6 mAdMSCs than SCID mAdMSCs. These findings implicate these two sets of proteins in the pathogenesis and maintenance of immunodeficiency.

Original languageEnglish
JournalInternational journal of molecular sciences
Volume20
Issue number11
DOIs
Publication statusPublished - May 30 2019

Fingerprint

Severe Combined Immunodeficiency
stem cells
Stem cells
Mesenchymal Stromal Cells
mice
Immunity
immunity
proteins
Proteins
HMGB1 Protein
Galectin 3
cells
Therapeutic Uses
Cell- and Tissue-Based Therapy
Tandem Mass Spectrometry
Inbred C57BL Mouse
Liquid Chromatography
pathogenesis
cytometry
Flow cytometry

Keywords

  • adult stem cells
  • mesenchymal stem cell
  • tissue regeneration, regenerative medicine

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice. / Nakashima, Yoshiki; Nahar, Saifun; Miyagi-Shiohira, Chika; Kinjo, Takao; Kobayashi, Naoya; Kitamura, Shinji; Saitoh, Issei; Watanabe, Masami; Fujita, Jiro; Noguchi, Hirofumi.

In: International journal of molecular sciences, Vol. 20, No. 11, 30.05.2019.

Research output: Contribution to journalArticle

Nakashima, Yoshiki ; Nahar, Saifun ; Miyagi-Shiohira, Chika ; Kinjo, Takao ; Kobayashi, Naoya ; Kitamura, Shinji ; Saitoh, Issei ; Watanabe, Masami ; Fujita, Jiro ; Noguchi, Hirofumi. / Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 11.
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