Identification of proteins associated with ion homeostasis and salt tolerance in barley

Dezhi Wu, Qiufang Shen, Long Qiu, Yong Han, Linzheng Ye, Zahra Jabeen, Qingyao Shu, Guoping Zhang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H+-ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.

Original languageEnglish
Pages (from-to)1381-1392
Number of pages12
JournalProteomics
Volume14
Issue number11
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Salt-Tolerance
Hordeum
Salinity
Homeostasis
Salts
Ions
Photosynthesis
Proteins
Oxidation-Reduction
Oxygenases
Proton-Translocating ATPases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Inductively coupled plasma
Tissue Plasminogen Activator
Calmodulin
Proteomics
Spectrometers
Genotype
Oxygen
Weights and Measures

Keywords

  • Hordeum vulgare and Hordeum spontaneum
  • Ion homeostasis
  • Plant proteomics
  • Salt tolerance

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Medicine(all)

Cite this

Wu, D., Shen, Q., Qiu, L., Han, Y., Ye, L., Jabeen, Z., ... Zhang, G. (2014). Identification of proteins associated with ion homeostasis and salt tolerance in barley. Proteomics, 14(11), 1381-1392. https://doi.org/10.1002/pmic.201300221

Identification of proteins associated with ion homeostasis and salt tolerance in barley. / Wu, Dezhi; Shen, Qiufang; Qiu, Long; Han, Yong; Ye, Linzheng; Jabeen, Zahra; Shu, Qingyao; Zhang, Guoping.

In: Proteomics, Vol. 14, No. 11, 2014, p. 1381-1392.

Research output: Contribution to journalArticle

Wu, D, Shen, Q, Qiu, L, Han, Y, Ye, L, Jabeen, Z, Shu, Q & Zhang, G 2014, 'Identification of proteins associated with ion homeostasis and salt tolerance in barley', Proteomics, vol. 14, no. 11, pp. 1381-1392. https://doi.org/10.1002/pmic.201300221
Wu, Dezhi ; Shen, Qiufang ; Qiu, Long ; Han, Yong ; Ye, Linzheng ; Jabeen, Zahra ; Shu, Qingyao ; Zhang, Guoping. / Identification of proteins associated with ion homeostasis and salt tolerance in barley. In: Proteomics. 2014 ; Vol. 14, No. 11. pp. 1381-1392.
@article{feee0815362b4f52ac2a7a4840a7f217,
title = "Identification of proteins associated with ion homeostasis and salt tolerance in barley",
abstract = "Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H+-ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.",
keywords = "Hordeum vulgare and Hordeum spontaneum, Ion homeostasis, Plant proteomics, Salt tolerance",
author = "Dezhi Wu and Qiufang Shen and Long Qiu and Yong Han and Linzheng Ye and Zahra Jabeen and Qingyao Shu and Guoping Zhang",
year = "2014",
doi = "10.1002/pmic.201300221",
language = "English",
volume = "14",
pages = "1381--1392",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "11",

}

TY - JOUR

T1 - Identification of proteins associated with ion homeostasis and salt tolerance in barley

AU - Wu, Dezhi

AU - Shen, Qiufang

AU - Qiu, Long

AU - Han, Yong

AU - Ye, Linzheng

AU - Jabeen, Zahra

AU - Shu, Qingyao

AU - Zhang, Guoping

PY - 2014

Y1 - 2014

N2 - Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H+-ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.

AB - Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H+-ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.

KW - Hordeum vulgare and Hordeum spontaneum

KW - Ion homeostasis

KW - Plant proteomics

KW - Salt tolerance

UR - http://www.scopus.com/inward/record.url?scp=84901832799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901832799&partnerID=8YFLogxK

U2 - 10.1002/pmic.201300221

DO - 10.1002/pmic.201300221

M3 - Article

VL - 14

SP - 1381

EP - 1392

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 11

ER -