Identification of osteopontin in isolated rabbit osteoclasts

Ken ichi Tezuka, Takuya Sato, Hiroshi Kamioka, Peter J. Nijweide, Kayo Tanaka, Tetsu Matsuo, Mitsue Ohta, Noriyoshi Kurihara, Yoshiyuki Hakeda, Masayoshi Kumegawa

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201 Citations (Scopus)

Abstract

Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines; however, little is known about protein factors produced by osteoclasts or bone-resorbing cells. A method utilizing the high affinity of osteoclasts for tissue culture dishes was developed to isolate a large number of pure osteoclasts from rabbit long bones. A cDNA library was then constructed from these isolated osteoclasts, and differential cDNA screening was performed between osteoclasts and spleen cells. Two clones representing osteoclast-specific clones, named OC-1 and OC-2, were isolated. By Northern blot analysis, OC-1 was expressed in osteoclasts and in kidneys, whereas OC-2 was specific for osteoclasts. OC-1 was found to encode osteopontin from its nucleotide sequence, and therefore, osteopontin may have other functions for osteoclastic bone resorption besides osteoclast attachment to bone.

Original languageEnglish
Pages (from-to)911-917
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number2
DOIs
Publication statusPublished - Jul 31 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Tezuka, K. I., Sato, T., Kamioka, H., Nijweide, P. J., Tanaka, K., Matsuo, T., Ohta, M., Kurihara, N., Hakeda, Y., & Kumegawa, M. (1992). Identification of osteopontin in isolated rabbit osteoclasts. Biochemical and Biophysical Research Communications, 186(2), 911-917. https://doi.org/10.1016/0006-291X(92)90832-6