Identification of Orchid fleck virus by reverse transcriptase-polymerase chain reaction and analysis of isolate relationships

A. L. Blanchfield, A. M. Mackenzie, A. Gibbs, H. Kondo, T. Tamada, C. R. Wilson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Using reverse transcription-polymerase chain reaction (RT-PCR) tests with a primer complementary to a region of its nucleoprotein gene together with a polydT/SP6 primer, Orchid fleck virus (OFV) was detected in 34 samples of infected orchids of several different genera from world-wide sources. The resulting DNA fragments were approximately 800 bp in length and their sequences were determined directly. Analysis of sequences of the major open reading frame (ORF) within the DNA fragments obtained showed the presence of two virus strains. The first group was of two isolates, the original Japanese isolate from which the primers were derived and one from Germany, and the second group contained 33 isolates from four continents. The sequences of different groups differed from one another by at least 15.6% (nucleotide) and 1.8% (amino-acid), but the within-group differences were much less (<1.7% difference). A search of the international nucleotide database with the OFV sequences showed them to be related, but distantly, to nucleoprotein regions of the genomes of four plant-infecting rhabdoviruses.

Original languageEnglish
Pages (from-to)713-718
Number of pages6
JournalJournal of Phytopathology
Volume149
Issue number11-12
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Orchid fleck virus
  • Orchids
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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