Identification of novel extracellular protein for PCB/biphenyl metabolism in Rhodococcus jostii RHA1

Yuki Atago, Jun Shimodaira, Naoto Araki, Nor'azizi Bin Othman, Zuriati Zakaria, Masao Fukuda, Junichiro Futami, Hirofumi Hara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rhodococcus jostii RHA1 (RHA1) degrades polychlorinated biphenyl (PCB) via co-metabolism with biphenyl. To identify the novel open reading frames (ORFs) that contribute to PCB/biphenyl metabolism in RHA1, we compared chromatin immunoprecipitation chip and transcriptomic data. Six novel ORFs involved in PCB/biphenyl metabolism were identified. Gene deletion mutants of these 6 ORFs were made and were tested for their ability to grow on biphenyl. Interestingly, only the ro10225 deletion mutant showed deficient growth on biphenyl. Analysis of Ro10225 protein function showed that growth of the ro10225 deletion mutant on biphenyl was recovered when exogenous recombinant Ro10225 protein was added to the culture medium. Although Ro10225 protein has no putative secretion signal sequence, partially degraded Ro10225 protein was detected in conditioned medium from wild-type RHA1 grown on biphenyl. This Ro10225 fragment appeared to form a complex with another PCB/biphenyl oxidation enzyme. These results indicated that Ro10225 protein is essential for the formation of the PCB/biphenyl dioxygenase complex in RHA1.

Original languageEnglish
Pages (from-to)1012-1019
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number5
DOIs
Publication statusPublished - May 2016

Fingerprint

Rhodococcus
Polychlorinated Biphenyls
Metabolism
Proteins
Open Reading Frames
diphenyl
Dioxygenases
Chromatin Immunoprecipitation
Gene Deletion
Conditioned Culture Medium
Growth
Protein Sorting Signals
Recombinant Proteins
Chromatin
Culture Media
Genes
Oxidation

Keywords

  • Biodegradation
  • Biphenyl
  • Polychlorinated biphenyl (PCB)
  • RHA1
  • Rhodococcus jostii

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Identification of novel extracellular protein for PCB/biphenyl metabolism in Rhodococcus jostii RHA1. / Atago, Yuki; Shimodaira, Jun; Araki, Naoto; Othman, Nor'azizi Bin; Zakaria, Zuriati; Fukuda, Masao; Futami, Junichiro; Hara, Hirofumi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 80, No. 5, 05.2016, p. 1012-1019.

Research output: Contribution to journalArticle

Atago, Yuki ; Shimodaira, Jun ; Araki, Naoto ; Othman, Nor'azizi Bin ; Zakaria, Zuriati ; Fukuda, Masao ; Futami, Junichiro ; Hara, Hirofumi. / Identification of novel extracellular protein for PCB/biphenyl metabolism in Rhodococcus jostii RHA1. In: Bioscience, Biotechnology and Biochemistry. 2016 ; Vol. 80, No. 5. pp. 1012-1019.
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