Identification of natural diterpenes that inhibit bacterial wilt disease in Tobacco, Tomato and Arabidopsis

Shigemi Seo, Kenji Gomi, Hisatoshi Kaku, Hiroshi Abe, Hideharu Seto, Shingo Nakatsu, Masahiro Neya, Michie Kobayashi, Kazuhiro Nakaho, Yuki Ichinose, Ichiro Mitsuhara, Yuko Ohashi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The soil-borne bacterial pathogen Ralstonia solanacearum invades a broad range of plants through their roots, resulting in wilting of the plant, but no effective protection against this disease has been developed. Two bacterial wilt disease-inhibiting compounds were biochemically isolated from tobacco and identified as sclareol and cis-abienol, labdane-type diterpenes. When exogenously applied to their roots, sclareol and cis-abienol inhibited wilt disease in tobacco, tomato and Arabidopsis plants without exhibiting any antibacterial activity. Microarray analysis identified many sclareol-responsive genes in Arabidopsis roots, including genes encoding or with a role in ATP-binding cassette (ABC) transporters, and biosynthesis and signaling of defense-related molecules and mitogen-activated protein kinase (MAPK) cascade components. Inhibition of wilt disease by sclareol was attenuated in Arabidopsis mutants defective in the ABC transporter AtPDR12, the MAPK MPK3, and ethylene and abscisic acid signaling pathways, and also in transgenic tobacco plants with reduced expression of NtPDR1, a tobacco homolog of AtPDR12. These results suggest that multiple host factors are involved in the inhibition of bacterial wilt disease by sclareol-related compounds.

Original languageEnglish
Pages (from-to)1432-1444
Number of pages13
JournalPlant and Cell Physiology
Volume53
Issue number8
DOIs
Publication statusPublished - Aug 2012

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Keywords

  • ABC transporter
  • Diterpene
  • Ralstonia solanacearum
  • Wilt disease

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Seo, S., Gomi, K., Kaku, H., Abe, H., Seto, H., Nakatsu, S., Neya, M., Kobayashi, M., Nakaho, K., Ichinose, Y., Mitsuhara, I., & Ohashi, Y. (2012). Identification of natural diterpenes that inhibit bacterial wilt disease in Tobacco, Tomato and Arabidopsis. Plant and Cell Physiology, 53(8), 1432-1444. https://doi.org/10.1093/pcp/pcs085