TY - JOUR
T1 - Identification of mutagenic heterocyclic amines (IQ, Trp-P-1 and AαC) in the water of the Danube River
AU - Kataoka, Hiroyuki
AU - Hayatsu, Toshiko
AU - Hietsch, Gerhard
AU - Steinkellner, Hans
AU - Nishioka, Sachiko
AU - Narimatsu, Shizuo
AU - Knasmüller, Siegfried
AU - Hayatsu, Hikoya
N1 - Funding Information:
The authors are thankful to W. Rodinger of the Austrian Bundesamt für Wasserwirtschaft for providing us with informations on the Danube water quality. This work was supported by the Grant-in-Aid for Cancer Research (8-5) from the Ministry of Health and Welfare, Japan.
PY - 2000/3/3
Y1 - 2000/3/3
N2 - Three mutagenic heterocyclic amines, 2-amino-3-methylimidazo-[4,5- f]quinoline (IQ), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-9H-pyrido[2,3-b]indole (AαC), were isolated and identified in water from the Danube River in Vienna. Heterocyclic amines were extracted from river water by the blue rayon hanging method, and analyzed by gas chromatography with a nitrogen-phosphorous detector (GC-NPD) and GC-mass spectrometry (GC-MS) after conversion into their N-dimethylaminomethylene derivatives. Identity of IQ, Trp-P-1 and AαC in the river water was confirmed by GC-MS. The contents of IQ, Trp-P-1 and AαC were estimated by GC-NPD at 1.78 ± 0.17, 0.14 ± 0.02 and 0.44 ± 0.02 ng/g blue rayon equivalent (n = 3), respectively. The total amounts of these amines accounted for 26% of the mutagenicity of blue rayon extracts evaluated by the Ames test using TA98 with metabolic activation. (C) 2000 Elsevier Science B.V.
AB - Three mutagenic heterocyclic amines, 2-amino-3-methylimidazo-[4,5- f]quinoline (IQ), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-9H-pyrido[2,3-b]indole (AαC), were isolated and identified in water from the Danube River in Vienna. Heterocyclic amines were extracted from river water by the blue rayon hanging method, and analyzed by gas chromatography with a nitrogen-phosphorous detector (GC-NPD) and GC-mass spectrometry (GC-MS) after conversion into their N-dimethylaminomethylene derivatives. Identity of IQ, Trp-P-1 and AαC in the river water was confirmed by GC-MS. The contents of IQ, Trp-P-1 and AαC were estimated by GC-NPD at 1.78 ± 0.17, 0.14 ± 0.02 and 0.44 ± 0.02 ng/g blue rayon equivalent (n = 3), respectively. The total amounts of these amines accounted for 26% of the mutagenicity of blue rayon extracts evaluated by the Ames test using TA98 with metabolic activation. (C) 2000 Elsevier Science B.V.
KW - Blue rayon
KW - Heterocyclic amines
KW - Mutagenicity
KW - River water
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U2 - 10.1016/S1383-5718(99)00235-1
DO - 10.1016/S1383-5718(99)00235-1
M3 - Article
C2 - 10751722
AN - SCOPUS:0034599151
VL - 466
SP - 27
EP - 35
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
SN - 1383-5718
IS - 1
ER -