Identification of mutagenic heterocyclic amines (IQ, Trp-P-1 and AαC) in the water of the Danube River

Hiroyuki Kataoka; Toshiko Hayatsu; Gerhard Hietsch; Hans Steinkellner; Sachiko Nishioka; Shizuo Narimatsu; Siegfried Knasmüller; Hikoya Hayatsu

doi:10.1016/S1383-5718(99)00235-1

Identification of mutagenic heterocyclic amines (IQ, Trp-P-1 and AαC) in the water of the Danube River

Hiroyuki Kataoka, Toshiko Hayatsu, Gerhard Hietsch, Hans Steinkellner, Sachiko Nishioka, Shizuo Narimatsu, Siegfried Knasmüller, Hikoya Hayatsu

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Three mutagenic heterocyclic amines, 2-amino-3-methylimidazo-[4,5- f]quinoline (IQ), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-9H-pyrido[2,3-b]indole (AαC), were isolated and identified in water from the Danube River in Vienna. Heterocyclic amines were extracted from river water by the blue rayon hanging method, and analyzed by gas chromatography with a nitrogen-phosphorous detector (GC-NPD) and GC-mass spectrometry (GC-MS) after conversion into their N-dimethylaminomethylene derivatives. Identity of IQ, Trp-P-1 and AαC in the river water was confirmed by GC-MS. The contents of IQ, Trp-P-1 and AαC were estimated by GC-NPD at 1.78 ± 0.17, 0.14 ± 0.02 and 0.44 ± 0.02 ng/g blue rayon equivalent (n = 3), respectively. The total amounts of these amines accounted for 26% of the mutagenicity of blue rayon extracts evaluated by the Ames test using TA98 with metabolic activation. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	27-35
Number of pages	9
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	466
Issue number	1
DOIs	https://doi.org/10.1016/S1383-5718(99)00235-1
Publication status	Published - Mar 3 2000

Keywords

Blue rayon
Heterocyclic amines
Mutagenicity
River water

ASJC Scopus subject areas

Genetics
Health, Toxicology and Mutagenesis

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Identification of mutagenic heterocyclic amines (IQ, Trp-P-1 and AαC) in the water of the Danube River. / Kataoka, Hiroyuki; Hayatsu, Toshiko; Hietsch, Gerhard; Steinkellner, Hans; Nishioka, Sachiko; Narimatsu, Shizuo; Knasmüller, Siegfried; Hayatsu, Hikoya.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 466, No. 1, 03.03.2000, p. 27-35.

Research output: Contribution to journal › Article › peer-review

Kataoka, Hiroyuki ; Hayatsu, Toshiko ; Hietsch, Gerhard ; Steinkellner, Hans ; Nishioka, Sachiko ; Narimatsu, Shizuo ; Knasmüller, Siegfried ; Hayatsu, Hikoya. / Identification of mutagenic heterocyclic amines (IQ, Trp-P-1 and AαC) in the water of the Danube River. In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2000 ; Vol. 466, No. 1. pp. 27-35.

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abstract = "Three mutagenic heterocyclic amines, 2-amino-3-methylimidazo-[4,5- f]quinoline (IQ), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-9H-pyrido[2,3-b]indole (AαC), were isolated and identified in water from the Danube River in Vienna. Heterocyclic amines were extracted from river water by the blue rayon hanging method, and analyzed by gas chromatography with a nitrogen-phosphorous detector (GC-NPD) and GC-mass spectrometry (GC-MS) after conversion into their N-dimethylaminomethylene derivatives. Identity of IQ, Trp-P-1 and AαC in the river water was confirmed by GC-MS. The contents of IQ, Trp-P-1 and AαC were estimated by GC-NPD at 1.78 ± 0.17, 0.14 ± 0.02 and 0.44 ± 0.02 ng/g blue rayon equivalent (n = 3), respectively. The total amounts of these amines accounted for 26% of the mutagenicity of blue rayon extracts evaluated by the Ames test using TA98 with metabolic activation. (C) 2000 Elsevier Science B.V.",

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AU - Steinkellner, Hans

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AU - Narimatsu, Shizuo

AU - Knasmüller, Siegfried

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