Abstract
Previously, we reported the structure of human L-histidine decarboxylase gene. To identify the regions that regulate the tissue-specific expression of HDC, we constructed a fusion DNA with the 5'-flanking region from -1003 to +99 of the HDC gene and chloramphenicol acetyltransferase (CAT) gene, which was then transfected into human basophilic leukemia KU-812-F cells or human epithelial carcinoma HeLa cells. The 1102 bp DNA fragment stimulated the CAT activity in KU-812-F cells, but not in HeLa cells. CAT analysis with a series of 5'-deletion constructs of the HDC-CAT gene revealed the existence of two positive and one negative regulatory elements at -855 to -841 and -532 to -497 and -829 to -821, respectively. Sequence analysis showed a nuclear factor c-Myb binding motif, TAACTG, at position -520. Gel mobility shift analysis showed that the nuclear extract of KU-812-F cells, but not that of HeLa cells, contains a factor which can bind to this motif. These results suggest that the 5'-flanking region of the HDC gene contains multiple regulatory elements for HDC gene expression and that at least one element, including a c-Myb binding motif, is responsible for the tissue-specific expression of HDC.
| Original language | English |
|---|---|
| Pages (from-to) | 935-940 |
| Number of pages | 6 |
| Journal | Journal of Biochemistry |
| Volume | 121 |
| Issue number | 5 |
| Publication status | Published - May 1997 |
| Externally published | Yes |
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Keywords
- c-Myb binding motif
- Human basophilic leukemia cells
- Human L-histidine decarboxylase gene
- Nuclear factor
- Regulatory element
ASJC Scopus subject areas
- Biochemistry
Cite this
Identification of multiple regulatory elements of human L-histidine decarboxylase gene. / Nakagawa, Satoshi; Okaya, Yuki; Yatsunami, Kimio; Tanaka, Satoshi; Ohtsu, Hiroshi; Fukui, Tetsuya; Watanabe, Takehiko; Ichikawa, Atsushi.
In: Journal of Biochemistry, Vol. 121, No. 5, 05.1997, p. 935-940.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of multiple regulatory elements of human L-histidine decarboxylase gene
AU - Nakagawa, Satoshi
AU - Okaya, Yuki
AU - Yatsunami, Kimio
AU - Tanaka, Satoshi
AU - Ohtsu, Hiroshi
AU - Fukui, Tetsuya
AU - Watanabe, Takehiko
AU - Ichikawa, Atsushi
PY - 1997/5
Y1 - 1997/5
N2 - Previously, we reported the structure of human L-histidine decarboxylase gene. To identify the regions that regulate the tissue-specific expression of HDC, we constructed a fusion DNA with the 5'-flanking region from -1003 to +99 of the HDC gene and chloramphenicol acetyltransferase (CAT) gene, which was then transfected into human basophilic leukemia KU-812-F cells or human epithelial carcinoma HeLa cells. The 1102 bp DNA fragment stimulated the CAT activity in KU-812-F cells, but not in HeLa cells. CAT analysis with a series of 5'-deletion constructs of the HDC-CAT gene revealed the existence of two positive and one negative regulatory elements at -855 to -841 and -532 to -497 and -829 to -821, respectively. Sequence analysis showed a nuclear factor c-Myb binding motif, TAACTG, at position -520. Gel mobility shift analysis showed that the nuclear extract of KU-812-F cells, but not that of HeLa cells, contains a factor which can bind to this motif. These results suggest that the 5'-flanking region of the HDC gene contains multiple regulatory elements for HDC gene expression and that at least one element, including a c-Myb binding motif, is responsible for the tissue-specific expression of HDC.
AB - Previously, we reported the structure of human L-histidine decarboxylase gene. To identify the regions that regulate the tissue-specific expression of HDC, we constructed a fusion DNA with the 5'-flanking region from -1003 to +99 of the HDC gene and chloramphenicol acetyltransferase (CAT) gene, which was then transfected into human basophilic leukemia KU-812-F cells or human epithelial carcinoma HeLa cells. The 1102 bp DNA fragment stimulated the CAT activity in KU-812-F cells, but not in HeLa cells. CAT analysis with a series of 5'-deletion constructs of the HDC-CAT gene revealed the existence of two positive and one negative regulatory elements at -855 to -841 and -532 to -497 and -829 to -821, respectively. Sequence analysis showed a nuclear factor c-Myb binding motif, TAACTG, at position -520. Gel mobility shift analysis showed that the nuclear extract of KU-812-F cells, but not that of HeLa cells, contains a factor which can bind to this motif. These results suggest that the 5'-flanking region of the HDC gene contains multiple regulatory elements for HDC gene expression and that at least one element, including a c-Myb binding motif, is responsible for the tissue-specific expression of HDC.
KW - c-Myb binding motif
KW - Human basophilic leukemia cells
KW - Human L-histidine decarboxylase gene
KW - Nuclear factor
KW - Regulatory element
UR - http://www.scopus.com/inward/record.url?scp=0031011273&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031011273&partnerID=8YFLogxK
M3 - Article
C2 - 9192736
AN - SCOPUS:0031011273
VL - 121
SP - 935
EP - 940
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 5
ER -