Identification of multiple regulatory elements of human L-histidine decarboxylase gene

Satoshi Nakagawa, Yuki Okaya, Kimio Yatsunami, Satoshi Tanaka, Hiroshi Ohtsu, Tetsuya Fukui, Takehiko Watanabe, Atsushi Ichikawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Previously, we reported the structure of human L-histidine decarboxylase gene. To identify the regions that regulate the tissue-specific expression of HDC, we constructed a fusion DNA with the 5'-flanking region from -1003 to +99 of the HDC gene and chloramphenicol acetyltransferase (CAT) gene, which was then transfected into human basophilic leukemia KU-812-F cells or human epithelial carcinoma HeLa cells. The 1102 bp DNA fragment stimulated the CAT activity in KU-812-F cells, but not in HeLa cells. CAT analysis with a series of 5'-deletion constructs of the HDC-CAT gene revealed the existence of two positive and one negative regulatory elements at -855 to -841 and -532 to -497 and -829 to -821, respectively. Sequence analysis showed a nuclear factor c-Myb binding motif, TAACTG, at position -520. Gel mobility shift analysis showed that the nuclear extract of KU-812-F cells, but not that of HeLa cells, contains a factor which can bind to this motif. These results suggest that the 5'-flanking region of the HDC gene contains multiple regulatory elements for HDC gene expression and that at least one element, including a c-Myb binding motif, is responsible for the tissue-specific expression of HDC.

Original languageEnglish
Pages (from-to)935-940
Number of pages6
JournalJournal of Biochemistry
Volume121
Issue number5
Publication statusPublished - May 1997
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Histidine
Chloramphenicol O-Acetyltransferase
Genes
HeLa Cells
5' Flanking Region
Tissue
DNA
Electrophoretic Mobility Shift Assay
Gene expression
Sequence Analysis
Leukemia
Fusion reactions
Gels
Carcinoma
Gene Expression

Keywords

  • c-Myb binding motif
  • Human basophilic leukemia cells
  • Human L-histidine decarboxylase gene
  • Nuclear factor
  • Regulatory element

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nakagawa, S., Okaya, Y., Yatsunami, K., Tanaka, S., Ohtsu, H., Fukui, T., ... Ichikawa, A. (1997). Identification of multiple regulatory elements of human L-histidine decarboxylase gene. Journal of Biochemistry, 121(5), 935-940.

Identification of multiple regulatory elements of human L-histidine decarboxylase gene. / Nakagawa, Satoshi; Okaya, Yuki; Yatsunami, Kimio; Tanaka, Satoshi; Ohtsu, Hiroshi; Fukui, Tetsuya; Watanabe, Takehiko; Ichikawa, Atsushi.

In: Journal of Biochemistry, Vol. 121, No. 5, 05.1997, p. 935-940.

Research output: Contribution to journalArticle

Nakagawa, S, Okaya, Y, Yatsunami, K, Tanaka, S, Ohtsu, H, Fukui, T, Watanabe, T & Ichikawa, A 1997, 'Identification of multiple regulatory elements of human L-histidine decarboxylase gene', Journal of Biochemistry, vol. 121, no. 5, pp. 935-940.
Nakagawa S, Okaya Y, Yatsunami K, Tanaka S, Ohtsu H, Fukui T et al. Identification of multiple regulatory elements of human L-histidine decarboxylase gene. Journal of Biochemistry. 1997 May;121(5):935-940.
Nakagawa, Satoshi ; Okaya, Yuki ; Yatsunami, Kimio ; Tanaka, Satoshi ; Ohtsu, Hiroshi ; Fukui, Tetsuya ; Watanabe, Takehiko ; Ichikawa, Atsushi. / Identification of multiple regulatory elements of human L-histidine decarboxylase gene. In: Journal of Biochemistry. 1997 ; Vol. 121, No. 5. pp. 935-940.
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