Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51

Jin Yan, Hidehito Kuroyanagi, Asato Kuroiwa, Yo Ichi Matsuda, Hiroshi Tokumitsu, Toshifumi Tomoda, Takuji Shirasawa, Masa Aki Muramatsu

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

A novel protein kinase related to the C. elegans serine/threonine kinase UNC-51 was cloned from mouse. The UNC-51-Like Kinase (ULK)1 is encoded by a cDNA of 1051 amino acids with calculated MW of 113 kDa. Comparison of the ULK1 and UNC-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (PS) domain and a conserved carboxyl-terminal (C) domain. ULK1 mRNA is expressed in various tissues, and is mapped to mouse chromosome 5F and rat chromosome 12q16.3, by fluorescent in situ hybridization. HA-tagged ULK1 is expressed as a protein of ~150 kDa in COS7 cells and is auto-phosphorylated in vitro in its PS domain. We propose that ULK1, UNC-51 and a yeast protein kinase Apg1p comprise a novel subfamily of protein kinase, which is structurally conserved among eukaryotes.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number1
DOIs
Publication statusPublished - May 8 1998
Externally publishedYes

Fingerprint

Protein Kinases
Chromosomes
Proline
Serine
Phosphotransferases
Fungal Proteins
Protein-Serine-Threonine Kinases
Eukaryota
Fluorescence In Situ Hybridization
Rats
Conservation
Complementary DNA
Tissue
Amino Acids
Messenger RNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51. / Yan, Jin; Kuroyanagi, Hidehito; Kuroiwa, Asato; Matsuda, Yo Ichi; Tokumitsu, Hiroshi; Tomoda, Toshifumi; Shirasawa, Takuji; Muramatsu, Masa Aki.

In: Biochemical and Biophysical Research Communications, Vol. 246, No. 1, 08.05.1998, p. 222-227.

Research output: Contribution to journalArticle

Yan, Jin ; Kuroyanagi, Hidehito ; Kuroiwa, Asato ; Matsuda, Yo Ichi ; Tokumitsu, Hiroshi ; Tomoda, Toshifumi ; Shirasawa, Takuji ; Muramatsu, Masa Aki. / Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 246, No. 1. pp. 222-227.
@article{f5494783fbd5471cbea9c9335d16a378,
title = "Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51",
abstract = "A novel protein kinase related to the C. elegans serine/threonine kinase UNC-51 was cloned from mouse. The UNC-51-Like Kinase (ULK)1 is encoded by a cDNA of 1051 amino acids with calculated MW of 113 kDa. Comparison of the ULK1 and UNC-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (PS) domain and a conserved carboxyl-terminal (C) domain. ULK1 mRNA is expressed in various tissues, and is mapped to mouse chromosome 5F and rat chromosome 12q16.3, by fluorescent in situ hybridization. HA-tagged ULK1 is expressed as a protein of ~150 kDa in COS7 cells and is auto-phosphorylated in vitro in its PS domain. We propose that ULK1, UNC-51 and a yeast protein kinase Apg1p comprise a novel subfamily of protein kinase, which is structurally conserved among eukaryotes.",
author = "Jin Yan and Hidehito Kuroyanagi and Asato Kuroiwa and Matsuda, {Yo Ichi} and Hiroshi Tokumitsu and Toshifumi Tomoda and Takuji Shirasawa and Muramatsu, {Masa Aki}",
year = "1998",
month = "5",
day = "8",
doi = "10.1006/bbrc.1998.8546",
language = "English",
volume = "246",
pages = "222--227",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51

AU - Yan, Jin

AU - Kuroyanagi, Hidehito

AU - Kuroiwa, Asato

AU - Matsuda, Yo Ichi

AU - Tokumitsu, Hiroshi

AU - Tomoda, Toshifumi

AU - Shirasawa, Takuji

AU - Muramatsu, Masa Aki

PY - 1998/5/8

Y1 - 1998/5/8

N2 - A novel protein kinase related to the C. elegans serine/threonine kinase UNC-51 was cloned from mouse. The UNC-51-Like Kinase (ULK)1 is encoded by a cDNA of 1051 amino acids with calculated MW of 113 kDa. Comparison of the ULK1 and UNC-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (PS) domain and a conserved carboxyl-terminal (C) domain. ULK1 mRNA is expressed in various tissues, and is mapped to mouse chromosome 5F and rat chromosome 12q16.3, by fluorescent in situ hybridization. HA-tagged ULK1 is expressed as a protein of ~150 kDa in COS7 cells and is auto-phosphorylated in vitro in its PS domain. We propose that ULK1, UNC-51 and a yeast protein kinase Apg1p comprise a novel subfamily of protein kinase, which is structurally conserved among eukaryotes.

AB - A novel protein kinase related to the C. elegans serine/threonine kinase UNC-51 was cloned from mouse. The UNC-51-Like Kinase (ULK)1 is encoded by a cDNA of 1051 amino acids with calculated MW of 113 kDa. Comparison of the ULK1 and UNC-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (PS) domain and a conserved carboxyl-terminal (C) domain. ULK1 mRNA is expressed in various tissues, and is mapped to mouse chromosome 5F and rat chromosome 12q16.3, by fluorescent in situ hybridization. HA-tagged ULK1 is expressed as a protein of ~150 kDa in COS7 cells and is auto-phosphorylated in vitro in its PS domain. We propose that ULK1, UNC-51 and a yeast protein kinase Apg1p comprise a novel subfamily of protein kinase, which is structurally conserved among eukaryotes.

UR - http://www.scopus.com/inward/record.url?scp=0032495976&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032495976&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.8546

DO - 10.1006/bbrc.1998.8546

M3 - Article

VL - 246

SP - 222

EP - 227

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -