Identification of mild freezing shock response pathways in barley based on transcriptome profiling

Xiaolei Wang, Dezhi Wu, Qian Yang, Jianbin Zeng, Gulei Jin, Zhong Hua Chen, Guoping Zhang, Fei Dai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Low temperature is a major abiotic stress affecting crop growth and productivity. A better understanding of low temperature tolerance mechanisms is imperative for developing the crop cultivars with improved tolerance. We herein performed an Illumina RNA-sequencing experiment using two barley genotypes differing in freezing tolerance (Nure, tolerant and Tremois, sensitive), to determine the transcriptome profiling and genotypic difference under mild freezing shock treatment after a very short acclimation for gene induction. A total of 6474 differentially expressed genes, almost evenly distributed on the seven chromosomes, were identified. The key DEGs could be classified into six signaling pathways, i.e., Ca2+ signaling, PtdOH signaling, CBFs pathway, ABA pathway, jasmonate pathway, and amylohydrolysis pathway. Expression values of DEGs in multiple signaling pathways were analyzed and a hypothetical model of mild freezing shock tolerance mechanism was proposed. Expression and sequence profile of HvCBFs cluster withinFrost resistance-H2, a major quantitative trait locus on 5H being closely related to low temperature tolerance in barley, were further illustrated, considering the crucial role ofHvCBFs on freezing tolerance. It may be concluded that multiple signaling pathways are activated in concert when barley is exposed to mild freezing shock. The pathway network we presented may provide a platform for further exploring the functions of genes involved in low temperature tolerance in barley.

Original languageEnglish
Article number106
JournalFrontiers in Plant Science
Volume7
Issue numberFEB2016
DOIs
Publication statusPublished - Feb 8 2016
Externally publishedYes

Fingerprint

transcriptomics
freezing
barley
cold tolerance
temperature
gene induction
crops
abiotic stress
quantitative trait loci
acclimation
genes
sequence analysis
chromosomes
calcium
genotype
cultivars

Keywords

  • Barley (Hordeum vulgare L.)
  • Differentially expressed genes
  • Freezing shock
  • RNA-sequencing
  • Signaling pathway

ASJC Scopus subject areas

  • Plant Science

Cite this

Identification of mild freezing shock response pathways in barley based on transcriptome profiling. / Wang, Xiaolei; Wu, Dezhi; Yang, Qian; Zeng, Jianbin; Jin, Gulei; Chen, Zhong Hua; Zhang, Guoping; Dai, Fei.

In: Frontiers in Plant Science, Vol. 7, No. FEB2016, 106, 08.02.2016.

Research output: Contribution to journalArticle

Wang, Xiaolei ; Wu, Dezhi ; Yang, Qian ; Zeng, Jianbin ; Jin, Gulei ; Chen, Zhong Hua ; Zhang, Guoping ; Dai, Fei. / Identification of mild freezing shock response pathways in barley based on transcriptome profiling. In: Frontiers in Plant Science. 2016 ; Vol. 7, No. FEB2016.
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