Identification of mammalian Pit-1w, possibly involved in spermatogenesis in mice

Shusuke Taniuchi, Kazuki Maeda, Toshiyuki Kudo, Sumio Takahashi, Sakae Takeuchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pit-1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals. Alternative splicing of Pit-1 gene transcripts has been shown to give rise to several variants with discrete transactivation properties; however, those arising from alternative promoters such as avian Pit-1w have not yet been identified in mammals. Here, comparative genomics analysis followed by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of 5′ cDNA ends (5′RACE) were used in identifying Pit-1w mRNA in the mouse pituitary. The mouse Pit-1w mRNA is generated by using an alternative promoter located in the first intron, as with chicken Pit-1w, and is expressed in a wide variety of tissues besides the pituitary. In the testis, Pit-1w is expressed as the predominant variant and a protein of 33 kDa. During the first wave of spermatogenesis, expression of Pit-1w mRNA at substantial levels was observed from 3 weeks, but not at 1 or 2 weeks after birth. A combination of immunohistochemistry and in situ hybridization detected Pit-1 mRNA and Pit-1 immunoreactivity in the spermatogonia, spermatocytes, and spermatids in the testis of adult mice. Because secondary spermatocytes and haploid spermatids increase in number between 18 and 20 days after birth in mice, it is possible that mouse Pit-1w plays a role in spermatogenesis. This is the first report demonstrating the expression of Pit-1 variants arising from alternative promoters in mammals.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume173
Issue number2
DOIs
Publication statusPublished - Sep 1 2011

Fingerprint

Spermatogenesis
spermatogenesis
mice
Mammals
Messenger RNA
Spermatocytes
Spermatids
spermatocytes
spermatids
promoter regions
mammals
Testis
testes
Parturition
Spermatogonia
spermatogonia
Pituitary Hormones
Haploidy
alternative splicing
Alternative Splicing

Keywords

  • Alternative promoter
  • Mouse
  • Pit-1
  • Testicular development
  • Variant

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification of mammalian Pit-1w, possibly involved in spermatogenesis in mice. / Taniuchi, Shusuke; Maeda, Kazuki; Kudo, Toshiyuki; Takahashi, Sumio; Takeuchi, Sakae.

In: General and Comparative Endocrinology, Vol. 173, No. 2, 01.09.2011, p. 289-294.

Research output: Contribution to journalArticle

@article{f72e5af11ace47889d604627d8169e78,
title = "Identification of mammalian Pit-1w, possibly involved in spermatogenesis in mice",
abstract = "Pit-1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals. Alternative splicing of Pit-1 gene transcripts has been shown to give rise to several variants with discrete transactivation properties; however, those arising from alternative promoters such as avian Pit-1w have not yet been identified in mammals. Here, comparative genomics analysis followed by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of 5′ cDNA ends (5′RACE) were used in identifying Pit-1w mRNA in the mouse pituitary. The mouse Pit-1w mRNA is generated by using an alternative promoter located in the first intron, as with chicken Pit-1w, and is expressed in a wide variety of tissues besides the pituitary. In the testis, Pit-1w is expressed as the predominant variant and a protein of 33 kDa. During the first wave of spermatogenesis, expression of Pit-1w mRNA at substantial levels was observed from 3 weeks, but not at 1 or 2 weeks after birth. A combination of immunohistochemistry and in situ hybridization detected Pit-1 mRNA and Pit-1 immunoreactivity in the spermatogonia, spermatocytes, and spermatids in the testis of adult mice. Because secondary spermatocytes and haploid spermatids increase in number between 18 and 20 days after birth in mice, it is possible that mouse Pit-1w plays a role in spermatogenesis. This is the first report demonstrating the expression of Pit-1 variants arising from alternative promoters in mammals.",
keywords = "Alternative promoter, Mouse, Pit-1, Testicular development, Variant",
author = "Shusuke Taniuchi and Kazuki Maeda and Toshiyuki Kudo and Sumio Takahashi and Sakae Takeuchi",
year = "2011",
month = "9",
day = "1",
doi = "10.1016/j.ygcen.2011.06.016",
language = "English",
volume = "173",
pages = "289--294",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Identification of mammalian Pit-1w, possibly involved in spermatogenesis in mice

AU - Taniuchi, Shusuke

AU - Maeda, Kazuki

AU - Kudo, Toshiyuki

AU - Takahashi, Sumio

AU - Takeuchi, Sakae

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Pit-1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals. Alternative splicing of Pit-1 gene transcripts has been shown to give rise to several variants with discrete transactivation properties; however, those arising from alternative promoters such as avian Pit-1w have not yet been identified in mammals. Here, comparative genomics analysis followed by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of 5′ cDNA ends (5′RACE) were used in identifying Pit-1w mRNA in the mouse pituitary. The mouse Pit-1w mRNA is generated by using an alternative promoter located in the first intron, as with chicken Pit-1w, and is expressed in a wide variety of tissues besides the pituitary. In the testis, Pit-1w is expressed as the predominant variant and a protein of 33 kDa. During the first wave of spermatogenesis, expression of Pit-1w mRNA at substantial levels was observed from 3 weeks, but not at 1 or 2 weeks after birth. A combination of immunohistochemistry and in situ hybridization detected Pit-1 mRNA and Pit-1 immunoreactivity in the spermatogonia, spermatocytes, and spermatids in the testis of adult mice. Because secondary spermatocytes and haploid spermatids increase in number between 18 and 20 days after birth in mice, it is possible that mouse Pit-1w plays a role in spermatogenesis. This is the first report demonstrating the expression of Pit-1 variants arising from alternative promoters in mammals.

AB - Pit-1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals. Alternative splicing of Pit-1 gene transcripts has been shown to give rise to several variants with discrete transactivation properties; however, those arising from alternative promoters such as avian Pit-1w have not yet been identified in mammals. Here, comparative genomics analysis followed by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of 5′ cDNA ends (5′RACE) were used in identifying Pit-1w mRNA in the mouse pituitary. The mouse Pit-1w mRNA is generated by using an alternative promoter located in the first intron, as with chicken Pit-1w, and is expressed in a wide variety of tissues besides the pituitary. In the testis, Pit-1w is expressed as the predominant variant and a protein of 33 kDa. During the first wave of spermatogenesis, expression of Pit-1w mRNA at substantial levels was observed from 3 weeks, but not at 1 or 2 weeks after birth. A combination of immunohistochemistry and in situ hybridization detected Pit-1 mRNA and Pit-1 immunoreactivity in the spermatogonia, spermatocytes, and spermatids in the testis of adult mice. Because secondary spermatocytes and haploid spermatids increase in number between 18 and 20 days after birth in mice, it is possible that mouse Pit-1w plays a role in spermatogenesis. This is the first report demonstrating the expression of Pit-1 variants arising from alternative promoters in mammals.

KW - Alternative promoter

KW - Mouse

KW - Pit-1

KW - Testicular development

KW - Variant

UR - http://www.scopus.com/inward/record.url?scp=79961127865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961127865&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2011.06.016

DO - 10.1016/j.ygcen.2011.06.016

M3 - Article

C2 - 21745476

AN - SCOPUS:79961127865

VL - 173

SP - 289

EP - 294

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -