Identification of maize silicon influx transporters

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Maize (Zea mays L.) shows a high accumulation of silicon (Si), but transporters involved in the uptake and distribution have not been identified. In the present study, we isolated two genes (ZmLsi1 and ZmLsi6), which are homologous to rice influx Si transporter OsLsi1. Heterologous expression in Xenopus laevis oocytes showed that both ZmLsi1 and ZmLsi6 are permeable to silicic acid. ZmLsi1 was mainly expressed in the roots. By contrast, ZmLsi6 was expressed more in the leaf sheaths and blades. Different from OsLsi1, the expression level of both ZmLsi1 and ZmLsi6 was unaffected by Si supply. Immunostaining showed that ZmLsi1 was localized on the plasma membrane of the distal side of root epidermal and hypodermal cells in the seminal and crown roots, and also in cortex cells in lateral roots. In the shoots, ZmLsi6 was found in the xylem parenchyma cells that are adjacent to the vessels in both leaf sheaths and leaf blades. ZmLsi6 in the leaf sheaths and blades also exhibited polar localization on the side facing towards the vessel. Taken together, it can be concluded that ZmLsi1 is an influx transporter of Si, which is responsible for the transport of Si from the external solution to the root cells and that ZmLsi6 mainly functions as a Si transporter for xylem unloading.

Original languageEnglish
Pages (from-to)5-12
Number of pages8
JournalPlant and Cell Physiology
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Silicon
silicon
Zea mays
transporters
corn
Xylem
xylem vessels
xylem
Silicic Acid
cells
leaves
silicic acid
Xenopus laevis
Crowns
leaf blade
parenchyma (plant tissue)
tree crown
Oocytes
cortex
oocytes

Keywords

  • Influx
  • Maize
  • NIP
  • Root
  • Silicon
  • Transporter

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Identification of maize silicon influx transporters. / Mitani, Namiki; Yamaji, Naoki; Ma, Jian Feng.

In: Plant and Cell Physiology, Vol. 50, No. 1, 01.2009, p. 5-12.

Research output: Contribution to journalArticle

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