TY - JOUR
T1 - Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut
AU - Han, Hongyan
AU - Wang, Chao
AU - Li, Yanbing
AU - Yu, Zhu
AU - Xu, Qingfang
AU - Li, Guangpeng
AU - Minh, Tang Thuy
AU - Nishino, Naoki
N1 - Funding Information:
Funding for this study was provided by ‘Inner Mongolia University Startup Foundation for Advanced Talents (Project No. 30500-5145140)’.
Publisher Copyright:
© 2017 Japanese Society of Animal Science.
PY - 2018/1
Y1 - 2018/1
N2 - In order to assess the survival of lactic acid bacteria (LAB) in whole crop maize silage in the gut of dairy cows, one representative silage sample and three different feces samples were collected from dairy cows on three dairy farms in Hua Bei, China and three dairy farms in Kyushu, Japan. The composition of the bacterial community was examined by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Lactobacillus acetotolerans was detected in all bunker-made maize silage samples, regardless of the dairy farm or sampling region from which they were sourced. A total of eight LAB species were detected in the maize silage samples, of which three (L. acetotolerans, L. pontis and L. casei) appeared to survive digestion. The populations of L. acetotolerans in silage and feces were 106–7 and 103–4 copies/g, respectively, indicating that, even for the LAB species showing potential survival in the gut, competition in this niche may be harsh and the population may substantially decrease during the digestion process. It may be difficult for silage LAB to survive in the gut of silage-fed dairy cows, because marked decrease in population can take place during the digestion process, even for surviving species.
AB - In order to assess the survival of lactic acid bacteria (LAB) in whole crop maize silage in the gut of dairy cows, one representative silage sample and three different feces samples were collected from dairy cows on three dairy farms in Hua Bei, China and three dairy farms in Kyushu, Japan. The composition of the bacterial community was examined by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Lactobacillus acetotolerans was detected in all bunker-made maize silage samples, regardless of the dairy farm or sampling region from which they were sourced. A total of eight LAB species were detected in the maize silage samples, of which three (L. acetotolerans, L. pontis and L. casei) appeared to survive digestion. The populations of L. acetotolerans in silage and feces were 106–7 and 103–4 copies/g, respectively, indicating that, even for the LAB species showing potential survival in the gut, competition in this niche may be harsh and the population may substantially decrease during the digestion process. It may be difficult for silage LAB to survive in the gut of silage-fed dairy cows, because marked decrease in population can take place during the digestion process, even for surviving species.
KW - dairy cows
KW - denaturing gradient gel electrophoresis
KW - lactic acid bacteria
KW - quantitative polymerase chain reaction
KW - silage
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U2 - 10.1111/asj.12896
DO - 10.1111/asj.12896
M3 - Article
C2 - 28884929
AN - SCOPUS:85029002219
VL - 89
SP - 97
EP - 104
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 1
ER -