Identification of individual barley chromosomes based on repetitive sequences: Conservative distribution of Afa-family repetitive sequences on the chromosomes of barley and wheat

Hisashi Tsujimoto, Yasuhiko Mukai, Keisuke Akagawa, Kiyotaka Nagaki, Junzo Fujigaki, Maki Yamamoto, Tetsuo Sasakuma

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The Afa-family repetitive sequences were isolated from barley (Hordeum vulgare, 2n = 14) and cloned as pHvA14. This sequence distinguished each barley chromosome by in situ hybridization. Double color fluorescence in situ hybridization using pHvA14 and 5S rDNA or HvRT-family sequence (subtelomeric sequence of barley) allocated individual barley chromosomes showing a specific pattern of pHvA14 to chromosome 1H to 7H. As the case of the D genome chromosomes of Aegilops squarrosa and common wheat (Triticum aestivum) hybridized by its Afa-family sequences, the signals of pHvA14 in barley chromosomes tended to appear in the distal regions that do not carry many chromosome band markers. In the telomeric regions these signals always placed in more proximal portions than those of HvRT-family. Based on the distribution patterns of Afa-family sequences in the chromosomes of barley and D genome chromosomes of wheat, we discuss a possible mechanism of amplification of the repetitive sequences during the evolution of Triticeae. In addition, we show here that HvRT-family also could be used to distinguish individual barley chromosomes from the patterns of in situ hybridization.

Original languageEnglish
Pages (from-to)303-309
Number of pages7
JournalGenes and Genetic Systems
Volume72
Issue number5
DOIs
Publication statusPublished - Oct 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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