Abstract
DNA fingerprinting method was applied to identify inbred strains of laboratory rats. By Southern blot hybridization with core sequence of minisatellite DNA as a probe, typical hypervariable patterns of DNA fingerprint were obtained in inbred rat strains. The patterns were completely different among 15 rat strains examined, and the patterns of the DNA fingerprint of samples obtained from the same strain were completely identical. The patterns of the DNA fingerprint of two substrains derived from the same strain were identical, indicating relative stability of the patterns over a large number of generations. Therefore, we concluded that the DNA fingerprinting method was useful for the identification of inbred strains in genetic monitoring of laboratory rats.
| Original language | English |
|---|---|
| Pages (from-to) | 603-606 |
| Number of pages | 4 |
| Journal | Laboratory Animal Science |
| Volume | 43 |
| Issue number | 6 |
| Publication status | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
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Identification of inbred rat strains by using DNA fingerprinting method. / Kunieda, Tetsuo; Nomura, N.; Ishizaki, R.; Araki, K.; Toyoda, Y.; Imamichi, T.
In: Laboratory Animal Science, Vol. 43, No. 6, 1993, p. 603-606.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of inbred rat strains by using DNA fingerprinting method
AU - Kunieda, Tetsuo
AU - Nomura, N.
AU - Ishizaki, R.
AU - Araki, K.
AU - Toyoda, Y.
AU - Imamichi, T.
PY - 1993
Y1 - 1993
N2 - DNA fingerprinting method was applied to identify inbred strains of laboratory rats. By Southern blot hybridization with core sequence of minisatellite DNA as a probe, typical hypervariable patterns of DNA fingerprint were obtained in inbred rat strains. The patterns were completely different among 15 rat strains examined, and the patterns of the DNA fingerprint of samples obtained from the same strain were completely identical. The patterns of the DNA fingerprint of two substrains derived from the same strain were identical, indicating relative stability of the patterns over a large number of generations. Therefore, we concluded that the DNA fingerprinting method was useful for the identification of inbred strains in genetic monitoring of laboratory rats.
AB - DNA fingerprinting method was applied to identify inbred strains of laboratory rats. By Southern blot hybridization with core sequence of minisatellite DNA as a probe, typical hypervariable patterns of DNA fingerprint were obtained in inbred rat strains. The patterns were completely different among 15 rat strains examined, and the patterns of the DNA fingerprint of samples obtained from the same strain were completely identical. The patterns of the DNA fingerprint of two substrains derived from the same strain were identical, indicating relative stability of the patterns over a large number of generations. Therefore, we concluded that the DNA fingerprinting method was useful for the identification of inbred strains in genetic monitoring of laboratory rats.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027762214&partnerID=8YFLogxK
M3 - Article
C2 - 8158988
AN - SCOPUS:0027762214
VL - 43
SP - 603
EP - 606
JO - Comparative Medicine
JF - Comparative Medicine
SN - 1532-0820
IS - 6
ER -