Identification of inbred rat strains by using DNA fingerprinting method

Tetsuo Kunieda, N. Nomura, R. Ishizaki, K. Araki, Y. Toyoda, T. Imamichi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

DNA fingerprinting method was applied to identify inbred strains of laboratory rats. By Southern blot hybridization with core sequence of minisatellite DNA as a probe, typical hypervariable patterns of DNA fingerprint were obtained in inbred rat strains. The patterns were completely different among 15 rat strains examined, and the patterns of the DNA fingerprint of samples obtained from the same strain were completely identical. The patterns of the DNA fingerprint of two substrains derived from the same strain were identical, indicating relative stability of the patterns over a large number of generations. Therefore, we concluded that the DNA fingerprinting method was useful for the identification of inbred strains in genetic monitoring of laboratory rats.

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalLaboratory Animal Science
Volume43
Issue number6
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Inbred Strains Rats
DNA Fingerprinting
DNA fingerprinting
Rats
DNA
rats
methodology
Minisatellite Repeats
Southern Blotting
minisatellite repeats
Southern blotting
hybridization
Monitoring
monitoring

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Kunieda, T., Nomura, N., Ishizaki, R., Araki, K., Toyoda, Y., & Imamichi, T. (1993). Identification of inbred rat strains by using DNA fingerprinting method. Laboratory Animal Science, 43(6), 603-606.

Identification of inbred rat strains by using DNA fingerprinting method. / Kunieda, Tetsuo; Nomura, N.; Ishizaki, R.; Araki, K.; Toyoda, Y.; Imamichi, T.

In: Laboratory Animal Science, Vol. 43, No. 6, 1993, p. 603-606.

Research output: Contribution to journalArticle

Kunieda, T, Nomura, N, Ishizaki, R, Araki, K, Toyoda, Y & Imamichi, T 1993, 'Identification of inbred rat strains by using DNA fingerprinting method', Laboratory Animal Science, vol. 43, no. 6, pp. 603-606.
Kunieda T, Nomura N, Ishizaki R, Araki K, Toyoda Y, Imamichi T. Identification of inbred rat strains by using DNA fingerprinting method. Laboratory Animal Science. 1993;43(6):603-606.
Kunieda, Tetsuo ; Nomura, N. ; Ishizaki, R. ; Araki, K. ; Toyoda, Y. ; Imamichi, T. / Identification of inbred rat strains by using DNA fingerprinting method. In: Laboratory Animal Science. 1993 ; Vol. 43, No. 6. pp. 603-606.
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