Identification of human skin from a tissue fragment by detection of squamous cell carcinoma-related antigen using an enzyme immunoassay

Takashi Kitao, Satoru Miyaishi, Yuji Yamamoto, Hideo Ishizu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new method of identifying human skin from a tissue fragment by detection of squamous cell carcinoma-related (SCC) antigen, using an enzyme immunoassay, was developed. When an extract was prepared from 0.1 g human skin homogenized with 1 ml of phosphate buffered saline, this method was able to detect SCC antigen in extracts diluted 102-fold. There was no difference in the detection limit between individuals. Species specificity was good, and there was no cross reaction observed with skins from animals. Our method could also discriminate between skin and other organs or tissues, except for esophagus and lung. A practical case to which this method was applied is presented.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalForensic Science International
Volume83
Issue number2
DOIs
Publication statusPublished - Dec 2 1996

Fingerprint

Forensic Anthropology
Immunoenzyme Techniques
Skin
Tissue
Enzymes
Species Specificity
Cross Reactions
Esophagus
Limit of Detection
Animals
Phosphates
Lung
squamous cell carcinoma-related antigen

Keywords

  • EIA
  • SCC antigen
  • Skin
  • Tissue fragment

ASJC Scopus subject areas

  • Genetics
  • Analytical Chemistry
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health

Cite this

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abstract = "A new method of identifying human skin from a tissue fragment by detection of squamous cell carcinoma-related (SCC) antigen, using an enzyme immunoassay, was developed. When an extract was prepared from 0.1 g human skin homogenized with 1 ml of phosphate buffered saline, this method was able to detect SCC antigen in extracts diluted 102-fold. There was no difference in the detection limit between individuals. Species specificity was good, and there was no cross reaction observed with skins from animals. Our method could also discriminate between skin and other organs or tissues, except for esophagus and lung. A practical case to which this method was applied is presented.",
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AU - Miyaishi, Satoru

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AU - Ishizu, Hideo

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AB - A new method of identifying human skin from a tissue fragment by detection of squamous cell carcinoma-related (SCC) antigen, using an enzyme immunoassay, was developed. When an extract was prepared from 0.1 g human skin homogenized with 1 ml of phosphate buffered saline, this method was able to detect SCC antigen in extracts diluted 102-fold. There was no difference in the detection limit between individuals. Species specificity was good, and there was no cross reaction observed with skins from animals. Our method could also discriminate between skin and other organs or tissues, except for esophagus and lung. A practical case to which this method was applied is presented.

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