Identification of hop polyphenolic components which inhibit prostaglandin E2 production by gingival epithelial cells stimulated with periodontal pathogen

Hiroaki Inaba, Motoyuki Tagashira, Daiki Honma, Tomomasa Kanda, Yurong Kou, Yasuyuki Ohtake, Atsuo Amano

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Chronic marginal periodontitis is a destructive inflammatory disease caused by an imbalance between bacterial virulence and host defense ability, resulting in eventual tooth exfoliation. Porphyromonas gingivalis, a major periodontal pathogen, triggers a series of cellular inflammatory responses including the production of prostaglandin E2 (PGE2), which causes periodontal destruction; thus, anti-inflammatory reagents are considered beneficial for periodontal therapy. In the present study, we examined whether hop- and apple-derived polyphenols (HBP and ACT, respectively) inhibit PGE 2 production by human gingival epithelial (HGE) cells stimulated with P. gingivalis components. HGE cells were stimulated with P. gingivalis membrane vesicles, and the effects of HBP, ACT and epigallocatechin gallate (EGCg) on PGE2 production by HGE cells were evaluated using an enzyme-linked immunosorbent assay. HBP and EGCg significantly inhibited PGE2 production, whereas ACT did not. By further fractionation steps of HBP to identify the effective components, 3 components of HBP, 2-[(2-methylpropanoyl)- phloroglucinol]1-O-β-D-glucopyranoside (MPPG), quercetin 3-O-β-D-glucopyranoside (isoquercitrin), and kaempferol 3-O-β-glucopyranoside (astragalin), were found to be elements which significantly inhibited cellular PGE2 production. These results suggest that HBP is a potent inhibitor of cellular PGE2 production induced by P. gingivalis, and HBP may be useful for the prevention and attenuation of periodontitis.

Original languageEnglish
Pages (from-to)527-530
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number3
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

Fingerprint

Humulus
Porphyromonas gingivalis
Dinoprostone
Epithelial Cells
Tooth Exfoliation
Phloroglucinol
Chronic Periodontitis
Periodontitis
Malus
Polyphenols
Prostaglandins E
Virulence
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Membranes

Keywords

  • Gingival epithelial cell
  • Hop bract polyphenol
  • Humulus lupulus L.
  • Porphyromonas gingivalis
  • Prostaglandin E

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Identification of hop polyphenolic components which inhibit prostaglandin E2 production by gingival epithelial cells stimulated with periodontal pathogen. / Inaba, Hiroaki; Tagashira, Motoyuki; Honma, Daiki; Kanda, Tomomasa; Kou, Yurong; Ohtake, Yasuyuki; Amano, Atsuo.

In: Biological and Pharmaceutical Bulletin, Vol. 31, No. 3, 03.2008, p. 527-530.

Research output: Contribution to journalArticle

Inaba, Hiroaki ; Tagashira, Motoyuki ; Honma, Daiki ; Kanda, Tomomasa ; Kou, Yurong ; Ohtake, Yasuyuki ; Amano, Atsuo. / Identification of hop polyphenolic components which inhibit prostaglandin E2 production by gingival epithelial cells stimulated with periodontal pathogen. In: Biological and Pharmaceutical Bulletin. 2008 ; Vol. 31, No. 3. pp. 527-530.
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