Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa

Michihito Sasaki, Masahiro Kajihara, Katendi Changula, Akina Mori-Kajihara, Hirohito Ogawa, Bernard M Hang'ombe, Aaron S Mweene, Martin Simuunza, Reiko Yoshida, Michael Carr, Yasuko Orba, Ayato Takada, Hirofumi Sawa

Research output: Contribution to journalArticle

Abstract

Group A rotavirus (RVA) is a major cause of diarrhea in children worldwide. Although RVA infects many animals, little is known about RVA in bats. The present study investigated the genetic diversity of RVA in Zambian bats. We identified RVA from two straw-colored fruit bats (Eidolon helvum) and an Egyptian fruit bat (Rousettus aegyptiacus), and analyzed the genome sequences of these strains. Genome segments of the RVA strains from Zambian E. helvum showed 97%-99% nucleotide sequence identity with those of other RVA strains from E. helvum in Cameroon, which is 2800 km from the sampling locations. These findings suggest that migratory straw-colored fruit bat species, distributed across sub-Saharan Africa, have the potential to disseminate RVA across long distances. By contrast, the RVA strain from Zambian R. aegyptiacus carried highly divergent NSP2 and NSP4 genes, leading us to propose novel genotypes N21 and E27, respectively. Notably, this RVA strain also shared the same genotype for VP6 and NSP3 with the RVA strains from Zambian E. helvum, suggesting interspecies transmission and genetic reassortment may have occurred between these two bat species in the past. Our study has important implications for RVA dispersal in bat populations, and expands our knowledge of the ecology, diversity and evolutionary relationships of RVA.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalInfection, Genetics and Evolution
Volume63
DOIs
Publication statusE-pub ahead of print - May 21 2018
Externally publishedYes

Fingerprint

Social Identification
Rotavirus
bat
Chiroptera
Fruit
fruit
fruits
straw
genotype
genome
Rousettus
Genotype
Genome
Cameroon
Sub-Saharan Africa
Africa
Africa South of the Sahara
Ecology
diarrhea
Diarrhea

Cite this

Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa. / Sasaki, Michihito; Kajihara, Masahiro; Changula, Katendi; Mori-Kajihara, Akina; Ogawa, Hirohito; Hang'ombe, Bernard M; Mweene, Aaron S; Simuunza, Martin; Yoshida, Reiko; Carr, Michael; Orba, Yasuko; Takada, Ayato; Sawa, Hirofumi.

In: Infection, Genetics and Evolution, Vol. 63, 21.05.2018, p. 104-109.

Research output: Contribution to journalArticle

Sasaki, M, Kajihara, M, Changula, K, Mori-Kajihara, A, Ogawa, H, Hang'ombe, BM, Mweene, AS, Simuunza, M, Yoshida, R, Carr, M, Orba, Y, Takada, A & Sawa, H 2018, 'Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa', Infection, Genetics and Evolution, vol. 63, pp. 104-109. https://doi.org/10.1016/j.meegid.2018.05.016
Sasaki, Michihito ; Kajihara, Masahiro ; Changula, Katendi ; Mori-Kajihara, Akina ; Ogawa, Hirohito ; Hang'ombe, Bernard M ; Mweene, Aaron S ; Simuunza, Martin ; Yoshida, Reiko ; Carr, Michael ; Orba, Yasuko ; Takada, Ayato ; Sawa, Hirofumi. / Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa. In: Infection, Genetics and Evolution. 2018 ; Vol. 63. pp. 104-109.
@article{c0ddb205e2114e92b57c839a2d1674c1,
title = "Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa",
abstract = "Group A rotavirus (RVA) is a major cause of diarrhea in children worldwide. Although RVA infects many animals, little is known about RVA in bats. The present study investigated the genetic diversity of RVA in Zambian bats. We identified RVA from two straw-colored fruit bats (Eidolon helvum) and an Egyptian fruit bat (Rousettus aegyptiacus), and analyzed the genome sequences of these strains. Genome segments of the RVA strains from Zambian E. helvum showed 97{\%}-99{\%} nucleotide sequence identity with those of other RVA strains from E. helvum in Cameroon, which is 2800 km from the sampling locations. These findings suggest that migratory straw-colored fruit bat species, distributed across sub-Saharan Africa, have the potential to disseminate RVA across long distances. By contrast, the RVA strain from Zambian R. aegyptiacus carried highly divergent NSP2 and NSP4 genes, leading us to propose novel genotypes N21 and E27, respectively. Notably, this RVA strain also shared the same genotype for VP6 and NSP3 with the RVA strains from Zambian E. helvum, suggesting interspecies transmission and genetic reassortment may have occurred between these two bat species in the past. Our study has important implications for RVA dispersal in bat populations, and expands our knowledge of the ecology, diversity and evolutionary relationships of RVA.",
author = "Michihito Sasaki and Masahiro Kajihara and Katendi Changula and Akina Mori-Kajihara and Hirohito Ogawa and Hang'ombe, {Bernard M} and Mweene, {Aaron S} and Martin Simuunza and Reiko Yoshida and Michael Carr and Yasuko Orba and Ayato Takada and Hirofumi Sawa",
note = "Copyright {\circledC} 2018 Elsevier B.V. All rights reserved.",
year = "2018",
month = "5",
day = "21",
doi = "10.1016/j.meegid.2018.05.016",
language = "English",
volume = "63",
pages = "104--109",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa

AU - Sasaki, Michihito

AU - Kajihara, Masahiro

AU - Changula, Katendi

AU - Mori-Kajihara, Akina

AU - Ogawa, Hirohito

AU - Hang'ombe, Bernard M

AU - Mweene, Aaron S

AU - Simuunza, Martin

AU - Yoshida, Reiko

AU - Carr, Michael

AU - Orba, Yasuko

AU - Takada, Ayato

AU - Sawa, Hirofumi

N1 - Copyright © 2018 Elsevier B.V. All rights reserved.

PY - 2018/5/21

Y1 - 2018/5/21

N2 - Group A rotavirus (RVA) is a major cause of diarrhea in children worldwide. Although RVA infects many animals, little is known about RVA in bats. The present study investigated the genetic diversity of RVA in Zambian bats. We identified RVA from two straw-colored fruit bats (Eidolon helvum) and an Egyptian fruit bat (Rousettus aegyptiacus), and analyzed the genome sequences of these strains. Genome segments of the RVA strains from Zambian E. helvum showed 97%-99% nucleotide sequence identity with those of other RVA strains from E. helvum in Cameroon, which is 2800 km from the sampling locations. These findings suggest that migratory straw-colored fruit bat species, distributed across sub-Saharan Africa, have the potential to disseminate RVA across long distances. By contrast, the RVA strain from Zambian R. aegyptiacus carried highly divergent NSP2 and NSP4 genes, leading us to propose novel genotypes N21 and E27, respectively. Notably, this RVA strain also shared the same genotype for VP6 and NSP3 with the RVA strains from Zambian E. helvum, suggesting interspecies transmission and genetic reassortment may have occurred between these two bat species in the past. Our study has important implications for RVA dispersal in bat populations, and expands our knowledge of the ecology, diversity and evolutionary relationships of RVA.

AB - Group A rotavirus (RVA) is a major cause of diarrhea in children worldwide. Although RVA infects many animals, little is known about RVA in bats. The present study investigated the genetic diversity of RVA in Zambian bats. We identified RVA from two straw-colored fruit bats (Eidolon helvum) and an Egyptian fruit bat (Rousettus aegyptiacus), and analyzed the genome sequences of these strains. Genome segments of the RVA strains from Zambian E. helvum showed 97%-99% nucleotide sequence identity with those of other RVA strains from E. helvum in Cameroon, which is 2800 km from the sampling locations. These findings suggest that migratory straw-colored fruit bat species, distributed across sub-Saharan Africa, have the potential to disseminate RVA across long distances. By contrast, the RVA strain from Zambian R. aegyptiacus carried highly divergent NSP2 and NSP4 genes, leading us to propose novel genotypes N21 and E27, respectively. Notably, this RVA strain also shared the same genotype for VP6 and NSP3 with the RVA strains from Zambian E. helvum, suggesting interspecies transmission and genetic reassortment may have occurred between these two bat species in the past. Our study has important implications for RVA dispersal in bat populations, and expands our knowledge of the ecology, diversity and evolutionary relationships of RVA.

U2 - 10.1016/j.meegid.2018.05.016

DO - 10.1016/j.meegid.2018.05.016

M3 - Article

C2 - 29792990

VL - 63

SP - 104

EP - 109

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -