Identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry

Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Chi L. Nguyen, Fumiko Taguchi, Kazuhiro Chiku, Tadashi Ishii, Hiroshi Ono, Mitsuru Yoshida, Yuki Ichinose

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and that glycosylation was required for virulence. We further identified some glycosylation-related genes, including vioA, vioB, vioT, fgt1, and fgt2. In this study, we newly identified vioR and vioM in a so-called viosamine island as biosynthetic genes for glycosylation of mVio in Pta 6605 by the mass spectrometry (MS) of flagellin glycan in the respective mutants. Furthermore, characterization of the mVio-related genes and MS analyses of flagellin glycans in other pathovars of P. syringae revealed that mVio-related genes were essential for mVio biosynthesis in flagellin glycans, and that P. syringae pv. syringae B728a, which does not possess a viosamine island, has a different structure of glycan in its flagellin protein.

Original languageEnglish
Pages (from-to)788-803
Number of pages16
JournalGenes
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 2011

Keywords

  • Flagellin
  • Glycosylation
  • Mass spectrometry
  • Viosamine island

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Yamamoto, M., Ohnishi-Kameyama, M., Nguyen, C. L., Taguchi, F., Chiku, K., Ishii, T., Ono, H., Yoshida, M., & Ichinose, Y. (2011). Identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry. Genes, 2(4), 788-803. https://doi.org/10.3390/genes2040788