Identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry

Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Chi L. Nguyen, Fumiko Taguchi, Kazuhiro Chiku, Tadashi Ishii, Hiroshi Ono, Mitsuru Yoshida, Yuki Ichinose

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and that glycosylation was required for virulence. We further identified some glycosylation-related genes, including vioA, vioB, vioT, fgt1, and fgt2. In this study, we newly identified vioR and vioM in a so-called viosamine island as biosynthetic genes for glycosylation of mVio in Pta 6605 by the mass spectrometry (MS) of flagellin glycan in the respective mutants. Furthermore, characterization of the mVio-related genes and MS analyses of flagellin glycans in other pathovars of P. syringae revealed that mVio-related genes were essential for mVio biosynthesis in flagellin glycans, and that P. syringae pv. syringae B728a, which does not possess a viosamine island, has a different structure of glycan in its flagellin protein.

Original languageEnglish
Pages (from-to)788-803
Number of pages16
JournalGenes
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Pseudomonas syringae
Flagellin
Glycosylation
Mass Spectrometry
Polysaccharides
Rhamnose
Genes
Islands
Trisaccharides
Essential Genes
Virulence
Proteins

Keywords

  • Flagellin
  • Glycosylation
  • Mass spectrometry
  • Viosamine island

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry. / Yamamoto, Masanobu; Ohnishi-Kameyama, Mayumi; Nguyen, Chi L.; Taguchi, Fumiko; Chiku, Kazuhiro; Ishii, Tadashi; Ono, Hiroshi; Yoshida, Mitsuru; Ichinose, Yuki.

In: Genes, Vol. 2, No. 4, 12.2011, p. 788-803.

Research output: Contribution to journalArticle

Yamamoto, M, Ohnishi-Kameyama, M, Nguyen, CL, Taguchi, F, Chiku, K, Ishii, T, Ono, H, Yoshida, M & Ichinose, Y 2011, 'Identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry', Genes, vol. 2, no. 4, pp. 788-803. https://doi.org/10.3390/genes2040788
Yamamoto, Masanobu ; Ohnishi-Kameyama, Mayumi ; Nguyen, Chi L. ; Taguchi, Fumiko ; Chiku, Kazuhiro ; Ishii, Tadashi ; Ono, Hiroshi ; Yoshida, Mitsuru ; Ichinose, Yuki. / Identification of genes involved in the glycosylation of modified viosamine of flagellins in pseudomonas syringae by mass spectrometry. In: Genes. 2011 ; Vol. 2, No. 4. pp. 788-803.
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