Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture

Bin Feng, Masakazu Morita, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We identified 22 genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (MSLC), among which Ser/Thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. We also revealed that aopk1 encodes a protein with an N-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in GenBank.

Original languageEnglish
Pages (from-to)470-473
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume102
Issue number5
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Aspergillus oryzae
Aspergillus
Cell culture
Genes
Proteins
Membranes
Phosphoprotein Phosphatases
Nucleic Acid Databases
Phosphatases
Liquids
Protein Kinases
Amino acids
Amino Acid Sequence
Amino Acids

Keywords

  • Aspergillus oryzae
  • differential genes
  • membrane-surface liquid culture
  • Ser/Thr protein kinase and phosphatase genes
  • subtractive hybridization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture. / Feng, Bin; Morita, Masakazu; Imanaka, Hiroyuki; Imamura, Koreyoshi; Nakanishi, Kazuhiro.

In: Journal of Bioscience and Bioengineering, Vol. 102, No. 5, 11.2006, p. 470-473.

Research output: Contribution to journalArticle

@article{9617250c12654a0cb57de1bc5f4054ba,
title = "Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture",
abstract = "We identified 22 genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (MSLC), among which Ser/Thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. We also revealed that aopk1 encodes a protein with an N-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in GenBank.",
keywords = "Aspergillus oryzae, differential genes, membrane-surface liquid culture, Ser/Thr protein kinase and phosphatase genes, subtractive hybridization",
author = "Bin Feng and Masakazu Morita and Hiroyuki Imanaka and Koreyoshi Imamura and Kazuhiro Nakanishi",
year = "2006",
month = "11",
doi = "10.1263/jbb.102.470",
language = "English",
volume = "102",
pages = "470--473",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture

AU - Feng, Bin

AU - Morita, Masakazu

AU - Imanaka, Hiroyuki

AU - Imamura, Koreyoshi

AU - Nakanishi, Kazuhiro

PY - 2006/11

Y1 - 2006/11

N2 - We identified 22 genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (MSLC), among which Ser/Thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. We also revealed that aopk1 encodes a protein with an N-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in GenBank.

AB - We identified 22 genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (MSLC), among which Ser/Thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. We also revealed that aopk1 encodes a protein with an N-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in GenBank.

KW - Aspergillus oryzae

KW - differential genes

KW - membrane-surface liquid culture

KW - Ser/Thr protein kinase and phosphatase genes

KW - subtractive hybridization

UR - http://www.scopus.com/inward/record.url?scp=33845636848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845636848&partnerID=8YFLogxK

U2 - 10.1263/jbb.102.470

DO - 10.1263/jbb.102.470

M3 - Article

C2 - 17189178

AN - SCOPUS:33845636848

VL - 102

SP - 470

EP - 473

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -