Abstract
To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.
| Original language | English |
|---|---|
| Pages (from-to) | 230-234 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 513 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Feb 27 2002 |
| Externally published | Yes |
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Keywords
- Brain
- cDNA microarray
- Differential display
- Honeybee
- Inositol 1,4,5-trisphosphate 5-phosphatase
- Mushroom body
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray. / Takeuchi, Hideaki; Fujiyuki, Tomoko; Shirai, Kenichi; Matsuo, Yuko; Kamikouchi, Azusa; Fujinawa, Yumi; Kato, Azusa; Tsujimoto, Atsumi; Kubo, Takeo.
In: FEBS Letters, Vol. 513, No. 2-3, 27.02.2002, p. 230-234.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray
AU - Takeuchi, Hideaki
AU - Fujiyuki, Tomoko
AU - Shirai, Kenichi
AU - Matsuo, Yuko
AU - Kamikouchi, Azusa
AU - Fujinawa, Yumi
AU - Kato, Azusa
AU - Tsujimoto, Atsumi
AU - Kubo, Takeo
PY - 2002/2/27
Y1 - 2002/2/27
N2 - To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.
AB - To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.
KW - Brain
KW - cDNA microarray
KW - Differential display
KW - Honeybee
KW - Inositol 1,4,5-trisphosphate 5-phosphatase
KW - Mushroom body
UR - http://www.scopus.com/inward/record.url?scp=0037181152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037181152&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(02)02319-0
DO - 10.1016/S0014-5793(02)02319-0
M3 - Article
C2 - 11904156
AN - SCOPUS:0037181152
VL - 513
SP - 230
EP - 234
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -