Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

Hideaki Takeuchi; Tomoko Fujiyuki; Kenichi Shirai; Yuko Matsuo; Azusa Kamikouchi; Yumi Fujinawa; Azusa Kato; Atsumi Tsujimoto; Takeo Kubo

doi:10.1016/S0014-5793(02)02319-0

Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

Hideaki Takeuchi, Tomoko Fujiyuki, Kenichi Shirai, Yuko Matsuo, Azusa Kamikouchi, Yumi Fujinawa, Azusa Kato, Atsumi Tsujimoto, Takeo Kubo

Research output: Contribution to journal › Article

44 Citations (Scopus)

Abstract

To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP₃) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP₃-mediated Ca²⁺ signaling is enhanced in these neurons.

Original language	English
Pages (from-to)	230-234
Number of pages	5
Journal	FEBS Letters
Volume	513
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(02)02319-0
Publication status	Published - Feb 27 2002

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Keywords

Brain
Differential display
Honeybee
Inositol 1,4,5-trisphosphate 5-phosphatase
Mushroom body
cDNA microarray

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Cite this

Takeuchi, H., Fujiyuki, T., Shirai, K., Matsuo, Y., Kamikouchi, A., Fujinawa, Y., Kato, A., Tsujimoto, A., & Kubo, T. (2002). Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray. FEBS Letters, 513(2-3), 230-234. https://doi.org/10.1016/S0014-5793(02)02319-0

Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray. / Takeuchi, Hideaki; Fujiyuki, Tomoko; Shirai, Kenichi; Matsuo, Yuko; Kamikouchi, Azusa; Fujinawa, Yumi; Kato, Azusa; Tsujimoto, Atsumi; Kubo, Takeo.

In: FEBS Letters, Vol. 513, No. 2-3, 27.02.2002, p. 230-234.

Research output: Contribution to journal › Article

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10.1016/S0014-5793(02)02319-0