Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

Hideaki Takeuchi; Tomoko Fujiyuki; Kenichi Shirai; Yuko Matsuo; Azusa Kamikouchi; Yumi Fujinawa; Azusa Kato; Atsumi Tsujimoto; Takeo Kubo

doi:10.1016/S0014-5793(02)02319-0

Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

Hideaki Takeuchi, Tomoko Fujiyuki, Kenichi Shirai, Yuko Matsuo, Azusa Kamikouchi, Yumi Fujinawa, Azusa Kato, Atsumi Tsujimoto, Takeo Kubo

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP₃) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP₃-mediated Ca²⁺ signaling is enhanced in these neurons.

Original language	English
Pages (from-to)	230-234
Number of pages	5
Journal	FEBS Letters
Volume	513
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(02)02319-0
Publication status	Published - Feb 27 2002
Externally published	Yes

Keywords

Brain
Differential display
Honeybee
Inositol 1,4,5-trisphosphate 5-phosphatase
Mushroom body
cDNA microarray

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)02319-0

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title = "Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray",

abstract = "To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.",

keywords = "Brain, Differential display, Honeybee, Inositol 1,4,5-trisphosphate 5-phosphatase, Mushroom body, cDNA microarray",

author = "Hideaki Takeuchi and Tomoko Fujiyuki and Kenichi Shirai and Yuko Matsuo and Azusa Kamikouchi and Yumi Fujinawa and Azusa Kato and Atsumi Tsujimoto and Takeo Kubo",

note = "Funding Information: This work was supported by Grants-in-Aid from the Bio-oriented Technology Research Advancement Institution (BRAIN), the Ministry of Education, Science, Sports, and Culture of Japan, and the Japan Society for the Promotion of Science (JSPS).",

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doi = "10.1016/S0014-5793(02)02319-0",

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T1 - Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

AU - Takeuchi, Hideaki

AU - Fujiyuki, Tomoko

AU - Shirai, Kenichi

AU - Matsuo, Yuko

AU - Kamikouchi, Azusa

AU - Fujinawa, Yumi

AU - Kato, Azusa

AU - Tsujimoto, Atsumi

AU - Kubo, Takeo

N1 - Funding Information: This work was supported by Grants-in-Aid from the Bio-oriented Technology Research Advancement Institution (BRAIN), the Ministry of Education, Science, Sports, and Culture of Japan, and the Japan Society for the Promotion of Science (JSPS).

PY - 2002/2/27

Y1 - 2002/2/27

N2 - To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.

AB - To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.

KW - Brain

KW - Differential display

KW - Honeybee

KW - Inositol 1,4,5-trisphosphate 5-phosphatase

KW - Mushroom body

KW - cDNA microarray

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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ER -

Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

Abstract

Keywords

ASJC Scopus subject areas

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