Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray

Hideaki Takeuchi, Tomoko Fujiyuki, Kenichi Shirai, Yuko Matsuo, Azusa Kamikouchi, Yumi Fujinawa, Azusa Kato, Atsumi Tsujimoto, Takeo Kubo

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP3) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP3-mediated Ca2+ signaling is enhanced in these neurons.

Original languageEnglish
Pages (from-to)230-234
Number of pages5
JournalFEBS Letters
Volume513
Issue number2-3
DOIs
Publication statusPublished - Feb 27 2002
Externally publishedYes

Fingerprint

Mushroom Bodies
Microarrays
Oligonucleotide Array Sequence Analysis
Complementary DNA
Genes
Display devices
Neurons
Inositol 1,4,5-Trisphosphate
Phosphoric Monoester Hydrolases
Social Class
Brain

Keywords

  • Brain
  • cDNA microarray
  • Differential display
  • Honeybee
  • Inositol 1,4,5-trisphosphate 5-phosphatase
  • Mushroom body

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray. / Takeuchi, Hideaki; Fujiyuki, Tomoko; Shirai, Kenichi; Matsuo, Yuko; Kamikouchi, Azusa; Fujinawa, Yumi; Kato, Azusa; Tsujimoto, Atsumi; Kubo, Takeo.

In: FEBS Letters, Vol. 513, No. 2-3, 27.02.2002, p. 230-234.

Research output: Contribution to journalArticle

Takeuchi, H, Fujiyuki, T, Shirai, K, Matsuo, Y, Kamikouchi, A, Fujinawa, Y, Kato, A, Tsujimoto, A & Kubo, T 2002, 'Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray', FEBS Letters, vol. 513, no. 2-3, pp. 230-234. https://doi.org/10.1016/S0014-5793(02)02319-0
Takeuchi, Hideaki ; Fujiyuki, Tomoko ; Shirai, Kenichi ; Matsuo, Yuko ; Kamikouchi, Azusa ; Fujinawa, Yumi ; Kato, Azusa ; Tsujimoto, Atsumi ; Kubo, Takeo. / Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray. In: FEBS Letters. 2002 ; Vol. 513, No. 2-3. pp. 230-234.
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