Identification of genes expressed during spore germination of Mycosphaerella pinodes

Hiroyuki Takahara, Kazuhiro Toyoda, Gento Tsuji, Yasuyuki Kubo, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalJournal of General Plant Pathology
Volume71
Issue number3
DOIs
Publication statusPublished - Jun 1 2005

Keywords

  • Mycosphaerella pinodes
  • Pisum sativum
  • Pycnospore germination
  • RT-PCR
  • Suppression subtractive hybridization (SSH)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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