Identification of essential amino acid residues of tRNA (Gm18)methyltransferase for methyl-transfer activity.

Kazunori Watanabe, H. Hori, Y. Endo

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Recent computation analyses have pointed out an existence of conserved amino acid motifs among RNA ribose 2'-O-methyltransferases. However, the functions of these motifs are unclear yet. We carried out the site-directed mutagenesis studies systematically on Thermus thermophilus HB8 tRNA (Gm18) methyltransferase gene. Subsequent biochemical analyses with purified variant enzymes clearly revealed that five conserved amino acid residues (Asn35, Arg41, Glu124, Ser150, and Asn152) are involved in S-adenosyl-L-methionine binding. This is for the first time reporting the function of the conserved motifs among RNA ribose 2'-O-methyltransferases.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages33-34
Number of pages2
Edition1
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

tRNA Methyltransferases
Essential Amino Acids
Ribose
Thermus thermophilus
S-Adenosylmethionine
Amino Acid Motifs
Site-Directed Mutagenesis
Amino Acids
Enzymes
Genes
RNA 2'-O-methyltransferase

Cite this

Watanabe, K., Hori, H., & Endo, Y. (2001). Identification of essential amino acid residues of tRNA (Gm18)methyltransferase for methyl-transfer activity. In Nucleic acids research. Supplement (2001) (1 ed., pp. 33-34)

Identification of essential amino acid residues of tRNA (Gm18)methyltransferase for methyl-transfer activity. / Watanabe, Kazunori; Hori, H.; Endo, Y.

Nucleic acids research. Supplement (2001). 1. ed. 2001. p. 33-34.

Research output: Chapter in Book/Report/Conference proceedingChapter

Watanabe, K, Hori, H & Endo, Y 2001, Identification of essential amino acid residues of tRNA (Gm18)methyltransferase for methyl-transfer activity. in Nucleic acids research. Supplement (2001). 1 edn, pp. 33-34.
Watanabe K, Hori H, Endo Y. Identification of essential amino acid residues of tRNA (Gm18)methyltransferase for methyl-transfer activity. In Nucleic acids research. Supplement (2001). 1 ed. 2001. p. 33-34
Watanabe, Kazunori ; Hori, H. ; Endo, Y. / Identification of essential amino acid residues of tRNA (Gm18)methyltransferase for methyl-transfer activity. Nucleic acids research. Supplement (2001). 1. ed. 2001. pp. 33-34
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