TY - JOUR
T1 - Identification of epstein-barr virus (EBV)-infected lymphocyte subtypes by how cytometric in situ hybridization in EBV-assodated lymphoproliferative diseases
AU - Kimura, Hiroshi
AU - Miyake, Kanae
AU - Yamauchi, Yohei
AU - Nishiyama, Kana
AU - Iwata, Seiko
AU - Iwatsuki, Keiji
AU - Gotoh, Kensei
AU - Seiji, Kojima
AU - Ito, Yoshinori
AU - Nishiyama, Yukihiro
N1 - Funding Information:
Received 3 March 2009; accepted 6 May 2009; electronically published 21 August 2009. Potential conflicts of interest: none reported. Financial support: Ministry of Education, Culture, Sports, Science and Technology, Japan (grant 19591247). Reprints or correspondence: Dr Kimura, Dept of Virology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466–8550, Japan (hkimura@med.nagoya-u.ac.jp).
PY - 2009/10/1
Y1 - 2009/10/1
N2 - To diagnose Epstein-Barr virus (EBV)-assodated diseases and to explore the pathogenesis of EBV infection, not only must the EBV load be measured, but EBV-infected cells must also be identified. We established a novel flow cytometric in situ hybridization assay to detect EBV+ suspension cells using a peptide nucleic acid probe specific for EBV-encoded small RNA (EBER). By enhancing fluorescence and photostability, we successfully stained EBER and surface antigens on the same cells. In 3 patients with hydroa vacciniforme-like lymphoproliferative disease, we demonstrated that 1.7%-25.9% of peripheral lymphocytes were infected with EBV and specifically identified these lymphocytes as CD3+CD4-CD8- y δ T cell receptor-positive T cells. The results indicate that this novel and noninvasive assay is a direct and reliable method of characterizing EBVinfected lymphocytes that can be used not only to diagnose EBV infection but also to clarify the pathogenesis of EBV-associated diseases.
AB - To diagnose Epstein-Barr virus (EBV)-assodated diseases and to explore the pathogenesis of EBV infection, not only must the EBV load be measured, but EBV-infected cells must also be identified. We established a novel flow cytometric in situ hybridization assay to detect EBV+ suspension cells using a peptide nucleic acid probe specific for EBV-encoded small RNA (EBER). By enhancing fluorescence and photostability, we successfully stained EBER and surface antigens on the same cells. In 3 patients with hydroa vacciniforme-like lymphoproliferative disease, we demonstrated that 1.7%-25.9% of peripheral lymphocytes were infected with EBV and specifically identified these lymphocytes as CD3+CD4-CD8- y δ T cell receptor-positive T cells. The results indicate that this novel and noninvasive assay is a direct and reliable method of characterizing EBVinfected lymphocytes that can be used not only to diagnose EBV infection but also to clarify the pathogenesis of EBV-associated diseases.
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U2 - 10.1086/605610
DO - 10.1086/605610
M3 - Article
C2 - 19698076
AN - SCOPUS:70349315348
SN - 0022-1899
VL - 200
SP - 1078
EP - 1087
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 7
ER -