Identification of epstein-barr virus (EBV)-infected lymphocyte subtypes by how cytometric in situ hybridization in EBV-assodated lymphoproliferative diseases

Hiroshi Kimura, Kanae Miyake, Yohei Yamauchi, Kana Nishiyama, Seiko Iwata, Keiji Iwatsuki, Kensei Gotoh, Kojima Seiji, Yoshinori Ito, Yukihiro Nishiyama

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40 Citations (Scopus)

Abstract

To diagnose Epstein-Barr virus (EBV)-assodated diseases and to explore the pathogenesis of EBV infection, not only must the EBV load be measured, but EBV-infected cells must also be identified. We established a novel flow cytometric in situ hybridization assay to detect EBV+ suspension cells using a peptide nucleic acid probe specific for EBV-encoded small RNA (EBER). By enhancing fluorescence and photostability, we successfully stained EBER and surface antigens on the same cells. In 3 patients with hydroa vacciniforme-like lymphoproliferative disease, we demonstrated that 1.7%-25.9% of peripheral lymphocytes were infected with EBV and specifically identified these lymphocytes as CD3+CD4-CD8- y δ T cell receptor-positive T cells. The results indicate that this novel and noninvasive assay is a direct and reliable method of characterizing EBVinfected lymphocytes that can be used not only to diagnose EBV infection but also to clarify the pathogenesis of EBV-associated diseases.

Original languageEnglish
Pages (from-to)1078-1087
Number of pages10
JournalJournal of Infectious Diseases
Volume200
Issue number7
DOIs
Publication statusPublished - Oct 1 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Kimura, H., Miyake, K., Yamauchi, Y., Nishiyama, K., Iwata, S., Iwatsuki, K., Gotoh, K., Seiji, K., Ito, Y., & Nishiyama, Y. (2009). Identification of epstein-barr virus (EBV)-infected lymphocyte subtypes by how cytometric in situ hybridization in EBV-assodated lymphoproliferative diseases. Journal of Infectious Diseases, 200(7), 1078-1087. https://doi.org/10.1086/605610