Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells

Michihide Shimono, Akiko Uenaka, Yuji Noguchi, Shuichiro Sato, Hideo Okumura, Kazuhiko Nakagawa, Katsuyuki Kiura, Mitsune Tanimoto, Eiichi Nakayama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We previously demonstrated a dominant IgG response against XAGE-1b antigen in a lung cancer patient by serological analysis of antigens by recombinant expression cloning (SEREX) analysis using a cDNA library from the autologous OU-LU-6 tumor cell line. In this study, we investigated recognition of XAGE-1b on OU-LU-6 tumor by the patient CD4-expressing tumor-infiltrating lymphocytes (CD4 TIL). The response of CD4 TIL obtained from malignant pleural effusion was determined against autologous OU-LU-6 tumor cells and XAGE-1b mRNA-transfected PHA-stimulated CD4 T-cells (T-APC) from healthy individuals sharing HLA-DR with the patient by performing IFNÁ secretion and ELISPOT assays. The patient CD4 TIL recognized OU-LU-6 in an HLA-DR-restricted manner and XAGE-1b mRNA-transfected T-APC derived from DRB1*0901-sharing healthy donor (HD)1 and HD2, but not DRB1*1101-sharing HD3 or HD4. Epitope analysis using 17 18-mer peptides with 12 overlapping amino acids revealed that the CD4 TIL recognized XAGE-1b 33-49. The findings suggest that the patient CD4 T-cells recognized the XAGE-1b 33-49-related epitope on autologous OU-LU-6 tumor cells in a manner restricted by DR*0901.

Original languageEnglish
Pages (from-to)835-840
Number of pages6
JournalInternational Journal of Oncology
Volume30
Issue number4
Publication statusPublished - Apr 2007

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Tumor-Infiltrating Lymphocytes
T-Lymphocytes
Antigens
HLA-DR Antigens
Epitopes
Malignant Pleural Effusion
Enzyme-Linked Immunospot Assay
Neoplasms
Messenger RNA
Tumor Cell Line
Gene Library
Organism Cloning
Lung Neoplasms
Immunoglobulin G
Adenocarcinoma of lung
Tissue Donors
Amino Acids
Peptides

Keywords

  • Cancer/testis antigen
  • CD4 T-cell response
  • XAGE

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Shimono, M., Uenaka, A., Noguchi, Y., Sato, S., Okumura, H., Nakagawa, K., ... Nakayama, E. (2007). Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells. International Journal of Oncology, 30(4), 835-840.

Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells. / Shimono, Michihide; Uenaka, Akiko; Noguchi, Yuji; Sato, Shuichiro; Okumura, Hideo; Nakagawa, Kazuhiko; Kiura, Katsuyuki; Tanimoto, Mitsune; Nakayama, Eiichi.

In: International Journal of Oncology, Vol. 30, No. 4, 04.2007, p. 835-840.

Research output: Contribution to journalArticle

Shimono, M, Uenaka, A, Noguchi, Y, Sato, S, Okumura, H, Nakagawa, K, Kiura, K, Tanimoto, M & Nakayama, E 2007, 'Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells', International Journal of Oncology, vol. 30, no. 4, pp. 835-840.
Shimono M, Uenaka A, Noguchi Y, Sato S, Okumura H, Nakagawa K et al. Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells. International Journal of Oncology. 2007 Apr;30(4):835-840.
Shimono, Michihide ; Uenaka, Akiko ; Noguchi, Yuji ; Sato, Shuichiro ; Okumura, Hideo ; Nakagawa, Kazuhiko ; Kiura, Katsuyuki ; Tanimoto, Mitsune ; Nakayama, Eiichi. / Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells. In: International Journal of Oncology. 2007 ; Vol. 30, No. 4. pp. 835-840.
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