Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama, Hiroshi Yamada, Mitsuko Hayashi, Toshihiko Oda, Akihito Yamaguchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

Original languageEnglish
Pages (from-to)57-60
Number of pages4
JournalNeuroscience Letters
Volume248
Issue number1
DOIs
Publication statusPublished - May 22 1998
Externally publishedYes

Fingerprint

D-Aspartic Acid
PC12 Cells
Pheochromocytoma
Endocrine Cells
Biosynthetic Pathways
Culture Media
Cultured Cells
High Pressure Liquid Chromatography

Keywords

  • D-Aspartate
  • De novo biosynthesis
  • Endocrine cell
  • High performance liquid chromatography
  • Immunohistochemistry
  • Mammalian cell
  • PC12 cells
  • Pineal gland

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of D-aspartate in rat pheochromocytoma PC12 cells. / Moriyama, Yoshinori; Yamada, Hiroshi; Hayashi, Mitsuko; Oda, Toshihiko; Yamaguchi, Akihito.

In: Neuroscience Letters, Vol. 248, No. 1, 22.05.1998, p. 57-60.

Research output: Contribution to journalArticle

Moriyama, Yoshinori ; Yamada, Hiroshi ; Hayashi, Mitsuko ; Oda, Toshihiko ; Yamaguchi, Akihito. / Identification of D-aspartate in rat pheochromocytoma PC12 cells. In: Neuroscience Letters. 1998 ; Vol. 248, No. 1. pp. 57-60.
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