Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama; Hiroshi Yamada; Mitsuko Hayashi; Toshihiko Oda; Akihito Yamaguchi

doi:10.1016/S0304-3940(98)00308-5

Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama, Hiroshi Yamada, Mitsuko Hayashi, Toshihiko Oda, Akihito Yamaguchi

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 10⁷ cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	248
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(98)00308-5
Publication status	Published - May 22 1998

Keywords

D-Aspartate
De novo biosynthesis
Endocrine cell
High performance liquid chromatography
Immunohistochemistry
Mammalian cell
PC12 cells
Pineal gland

ASJC Scopus subject areas

Neuroscience(all)

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title = "Identification of D-aspartate in rat pheochromocytoma PC12 cells",

abstract = "D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.",

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AU - Yamada, Hiroshi

AU - Hayashi, Mitsuko

AU - Oda, Toshihiko

AU - Yamaguchi, Akihito

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N2 - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

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