Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama; Hiroshi Yamada; Mitsuko Hayashi; Toshihiko Oda; Akihito Yamaguchi

doi:10.1016/S0304-3940(98)00308-5

Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama, Hiroshi Yamada, Mitsuko Hayashi, Toshihiko Oda, Akihito Yamaguchi

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 10⁷ cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	248
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(98)00308-5
Publication status	Published - May 22 1998

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Keywords

D-Aspartate
De novo biosynthesis
Endocrine cell
High performance liquid chromatography
Immunohistochemistry
Mammalian cell
PC12 cells
Pineal gland

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Moriyama, Y., Yamada, H., Hayashi, M., Oda, T., & Yamaguchi, A. (1998). Identification of D-aspartate in rat pheochromocytoma PC12 cells. Neuroscience Letters, 248(1), 57-60. https://doi.org/10.1016/S0304-3940(98)00308-5

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Access to Document

10.1016/S0304-3940(98)00308-5