Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama; Hiroshi Yamada; Mitsuko Hayashi; Toshihiko Oda; Akihito Yamaguchi

doi:10.1016/S0304-3940(98)00308-5

Identification of D-aspartate in rat pheochromocytoma PC12 cells

Yoshinori Moriyama, Hiroshi Yamada, Mitsuko Hayashi, Toshihiko Oda, Akihito Yamaguchi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 10⁷ cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	248
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(98)00308-5
Publication status	Published - May 22 1998
Externally published	Yes

Keywords

D-Aspartate
De novo biosynthesis
Endocrine cell
High performance liquid chromatography
Immunohistochemistry
Mammalian cell
PC12 cells
Pineal gland

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(98)00308-5

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title = "Identification of D-aspartate in rat pheochromocytoma PC12 cells",

abstract = "D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.",

keywords = "D-Aspartate, De novo biosynthesis, Endocrine cell, High performance liquid chromatography, Immunohistochemistry, Mammalian cell, PC12 cells, Pineal gland",

author = "Yoshinori Moriyama and Hiroshi Yamada and Mitsuko Hayashi and Toshihiko Oda and Akihito Yamaguchi",

note = "Funding Information: We wish to thank Professor K. Tanizawa (Osaka University, ISIR) for the valuable discussion. H.Y. was supported by a Research Fellowship from the Japan Society from Promotion of Science for Young Scientists. This work was supported in part by grants from the Japanese Ministry of Education, Science and Culture, the Terumo Science Foundation, the Chiba-Geigy Foundation (Japan) for the Promotion of Science, and the Takeda Science Foundation. ",

year = "1998",

month = may,

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doi = "10.1016/S0304-3940(98)00308-5",

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AU - Moriyama, Yoshinori

AU - Yamada, Hiroshi

AU - Hayashi, Mitsuko

AU - Oda, Toshihiko

AU - Yamaguchi, Akihito

N1 - Funding Information: We wish to thank Professor K. Tanizawa (Osaka University, ISIR) for the valuable discussion. H.Y. was supported by a Research Fellowship from the Japan Society from Promotion of Science for Young Scientists. This work was supported in part by grants from the Japanese Ministry of Education, Science and Culture, the Terumo Science Foundation, the Chiba-Geigy Foundation (Japan) for the Promotion of Science, and the Takeda Science Foundation.

PY - 1998/5/22

Y1 - 1998/5/22

N2 - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

AB - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.

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KW - De novo biosynthesis

KW - Endocrine cell

KW - High performance liquid chromatography

KW - Immunohistochemistry

KW - Mammalian cell

KW - PC12 cells

KW - Pineal gland

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Identification of D-aspartate in rat pheochromocytoma PC12 cells

Abstract

Keywords

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