Abstract
D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
Original language | English |
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Pages (from-to) | 57-60 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 248 |
Issue number | 1 |
DOIs | |
Publication status | Published - May 22 1998 |
Externally published | Yes |
Keywords
- D-Aspartate
- De novo biosynthesis
- Endocrine cell
- High performance liquid chromatography
- Immunohistochemistry
- Mammalian cell
- PC12 cells
- Pineal gland
ASJC Scopus subject areas
- Neuroscience(all)