TY - JOUR
T1 - Identification of D-aspartate in rat pheochromocytoma PC12 cells
AU - Moriyama, Yoshinori
AU - Yamada, Hiroshi
AU - Hayashi, Mitsuko
AU - Oda, Toshihiko
AU - Yamaguchi, Akihito
PY - 1998/5/22
Y1 - 1998/5/22
N2 - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
AB - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
KW - D-Aspartate
KW - De novo biosynthesis
KW - Endocrine cell
KW - High performance liquid chromatography
KW - Immunohistochemistry
KW - Mammalian cell
KW - PC12 cells
KW - Pineal gland
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U2 - 10.1016/S0304-3940(98)00308-5
DO - 10.1016/S0304-3940(98)00308-5
M3 - Article
C2 - 9665663
AN - SCOPUS:0032557514
VL - 248
SP - 57
EP - 60
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -