Abstract
D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
| Original language | English |
|---|---|
| Pages (from-to) | 57-60 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 248 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 22 1998 |
| Externally published | Yes |
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Keywords
- D-Aspartate
- De novo biosynthesis
- Endocrine cell
- High performance liquid chromatography
- Immunohistochemistry
- Mammalian cell
- PC12 cells
- Pineal gland
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Identification of D-aspartate in rat pheochromocytoma PC12 cells. / Moriyama, Yoshinori; Yamada, Hiroshi; Hayashi, Mitsuko; Oda, Toshihiko; Yamaguchi, Akihito.
In: Neuroscience Letters, Vol. 248, No. 1, 22.05.1998, p. 57-60.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of D-aspartate in rat pheochromocytoma PC12 cells
AU - Moriyama, Yoshinori
AU - Yamada, Hiroshi
AU - Hayashi, Mitsuko
AU - Oda, Toshihiko
AU - Yamaguchi, Akihito
PY - 1998/5/22
Y1 - 1998/5/22
N2 - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
AB - D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
KW - D-Aspartate
KW - De novo biosynthesis
KW - Endocrine cell
KW - High performance liquid chromatography
KW - Immunohistochemistry
KW - Mammalian cell
KW - PC12 cells
KW - Pineal gland
UR - http://www.scopus.com/inward/record.url?scp=0032557514&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032557514&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(98)00308-5
DO - 10.1016/S0304-3940(98)00308-5
M3 - Article
C2 - 9665663
AN - SCOPUS:0032557514
VL - 248
SP - 57
EP - 60
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -