Abstract
D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 107 cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D- aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
| Original language | English |
|---|---|
| Pages (from-to) | 57-60 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 248 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 22 1998 |
Keywords
- D-Aspartate
- De novo biosynthesis
- Endocrine cell
- High performance liquid chromatography
- Immunohistochemistry
- Mammalian cell
- PC12 cells
- Pineal gland
ASJC Scopus subject areas
- Neuroscience(all)
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Moriyama, Y., Yamada, H., Hayashi, M., Oda, T., & Yamaguchi, A. (1998). Identification of D-aspartate in rat pheochromocytoma PC12 cells. Neuroscience Letters, 248(1), 57-60. https://doi.org/10.1016/S0304-3940(98)00308-5