Identification of cis-elements regulating expression of Fgf10 during limb development

Hidemi Sasaki, Takashi Yamaoka, Hideyo Ohuchi, Akihiro Yasue, Tsutomu Nohno, Hirotaka Kawano, Shigeaki Kato, Mitsuo Itakura, Masaru Nagayama, Sumihare Noji

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fibroblast growth factor 10 (FGF10) is known to be expressed in limb mesenchymal cells and to function as a mesenchymal signaling factor involved in epithelial-mesenchymal interactions during limb development. To elucidate regulation of FgflO expression expression, we isolated the promoter region of FgflO containing its 2.0 kb upstream 5'-fragment from the initiation codon and its 0.9 kb downstream fragment. Transcriptional activity of the fragment was examined with transgenic mice, using a lacZ-reporter system. Although no significant expression of the reporter gene was observed for the 0.2 kb 5'-fragment, expression was detected in the apical ectodermal ridge of the limb bud and developing cartilage of the limb for the 2.0 kb and 0.7 kb 5'-fragments, respectively. From comparison of the mouse sequences of the 2.0 kb fragment with corresponding sequences of human and chicken FgflO, we identified 17 conserved putative enhancer motifs for AER expression and other unidentified expressions. For limb cartilage expression, we found putative enhancer sequences conserved among the three species in the 0.7 kb 5'-fragment. In the fragment, three DNA binding motifs were identified in the mouse and human sequence, although they are not conserved in the corresponding chicken sequence.

Original languageEnglish
Pages (from-to)963-967
Number of pages5
JournalInternational Journal of Developmental Biology
Volume46
Issue number7
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Extremities
Cartilage
Chickens
Fibroblast Growth Factor 10
Limb Buds
Nucleotide Motifs
Initiator Codon
Reporter Genes
Genetic Promoter Regions
Transgenic Mice

Keywords

  • Developing cartilage
  • Enhancer analysis
  • Fgfl0
  • Mouse
  • Transgenic mouse

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Sasaki, H., Yamaoka, T., Ohuchi, H., Yasue, A., Nohno, T., Kawano, H., ... Noji, S. (2002). Identification of cis-elements regulating expression of Fgf10 during limb development. International Journal of Developmental Biology, 46(7), 963-967.

Identification of cis-elements regulating expression of Fgf10 during limb development. / Sasaki, Hidemi; Yamaoka, Takashi; Ohuchi, Hideyo; Yasue, Akihiro; Nohno, Tsutomu; Kawano, Hirotaka; Kato, Shigeaki; Itakura, Mitsuo; Nagayama, Masaru; Noji, Sumihare.

In: International Journal of Developmental Biology, Vol. 46, No. 7, 2002, p. 963-967.

Research output: Contribution to journalArticle

Sasaki, H, Yamaoka, T, Ohuchi, H, Yasue, A, Nohno, T, Kawano, H, Kato, S, Itakura, M, Nagayama, M & Noji, S 2002, 'Identification of cis-elements regulating expression of Fgf10 during limb development', International Journal of Developmental Biology, vol. 46, no. 7, pp. 963-967.
Sasaki, Hidemi ; Yamaoka, Takashi ; Ohuchi, Hideyo ; Yasue, Akihiro ; Nohno, Tsutomu ; Kawano, Hirotaka ; Kato, Shigeaki ; Itakura, Mitsuo ; Nagayama, Masaru ; Noji, Sumihare. / Identification of cis-elements regulating expression of Fgf10 during limb development. In: International Journal of Developmental Biology. 2002 ; Vol. 46, No. 7. pp. 963-967.
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