Identification of chick rax/rx genes with overlapping patterns of expression during early eye and brain development

Hideyo Ohuchi, Sayuri Tomonari, Hiroyuki Itoh, Takashi Mikawa, Sumihare Noji

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We have isolated chick rax/rx cDNAs, cRaxL (chick Rax/Rx-like) and cRax. (chick Rax) and examined their expression patterns during early eye and brain development. The cRaxL cDNA encodes a 228 amino acid protein that is most closely related to the zebrafish Rx1 and Rx2. The cRax cDNA encodes a 317 amino acid protein, which shares higher homology with the Xenopus Rx. In addition to the homeodomain, the octapeptide and paired tail domains are conserved between the cRax and other vertebrate Rax/Rx, while cRaxL lacks the octapeptide containing N-terminal region which is conserved among all other members of the rax/rx gene family identified so far. The chick rax/rx genes are expressed in overlapping domains in the anterior neural ectoderm which corresponds to the forebrain and retina field, and later in the optic vesicle, cRax mRNA can be detected earlier than cRaxL prior to the formation of the notochord and its expression domain appears broader than that of cRaxL.

Original languageEnglish
Pages (from-to)193-195
Number of pages3
JournalMechanisms of Development
Volume85
Issue number1-2
DOIs
Publication statusPublished - Jul 1 1999
Externally publishedYes

Fingerprint

Overlapping Genes
Complementary DNA
Brain
Notochord
Amino Acids
Ectoderm
Zebrafish
Prosencephalon
Xenopus
Genes
Vertebrates
Retina
Tail
Proteins
Messenger RNA

Keywords

  • Brain
  • Chick
  • Development
  • Eye
  • rax
  • Retina field
  • rx

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Identification of chick rax/rx genes with overlapping patterns of expression during early eye and brain development. / Ohuchi, Hideyo; Tomonari, Sayuri; Itoh, Hiroyuki; Mikawa, Takashi; Noji, Sumihare.

In: Mechanisms of Development, Vol. 85, No. 1-2, 01.07.1999, p. 193-195.

Research output: Contribution to journalArticle

Ohuchi, Hideyo ; Tomonari, Sayuri ; Itoh, Hiroyuki ; Mikawa, Takashi ; Noji, Sumihare. / Identification of chick rax/rx genes with overlapping patterns of expression during early eye and brain development. In: Mechanisms of Development. 1999 ; Vol. 85, No. 1-2. pp. 193-195.
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