Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Tuoya, Koichi Hirayama, Tadahiro Nagaoka, Dongwei Yu, Takayuki Fukuda, Hiroko Tada, Hidenori Yamada, Masaharu Seno

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

Original languageEnglish
Pages (from-to)263-268
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume334
Issue number1
DOIs
Publication statusPublished - Aug 19 2005

Fingerprint

Microarrays
Oligonucleotide Array Sequence Analysis
Glass
DNA
Gene expression
Interleukin-6 Receptors
Diamond
Fibroblasts
BALB 3T3 Cells
Assays
Carbon
Microarray Analysis
Transcriptome
Biological Assay
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Gene Expression

Keywords

  • BALB/c 3T3 cells
  • Cell surface marker
  • DLC-coated glass
  • DNA microarray
  • IL-6 receptor α
  • SV-T2 cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass. / Tuoya; Hirayama, Koichi; Nagaoka, Tadahiro; Yu, Dongwei; Fukuda, Takayuki; Tada, Hiroko; Yamada, Hidenori; Seno, Masaharu.

In: Biochemical and Biophysical Research Communications, Vol. 334, No. 1, 19.08.2005, p. 263-268.

Research output: Contribution to journalArticle

Tuoya ; Hirayama, Koichi ; Nagaoka, Tadahiro ; Yu, Dongwei ; Fukuda, Takayuki ; Tada, Hiroko ; Yamada, Hidenori ; Seno, Masaharu. / Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 334, No. 1. pp. 263-268.
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