Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Tuoya; Koichi Hirayama; Tadahiro Nagaoka; Dongwei Yu; Takayuki Fukuda; Hiroko Tada; Hidenori Yamada; Masaharu Seno

doi:10.1016/j.bbrc.2005.06.083

Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Tuoya, Koichi Hirayama, Tadahiro Nagaoka, Dongwei Yu, Takayuki Fukuda, Hiroko Tada, Hidenori Yamada, Masaharu Seno

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

Original language	English
Pages (from-to)	263-268
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	334
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.06.083
Publication status	Published - Aug 19 2005

Keywords

BALB/c 3T3 cells
Cell surface marker
DLC-coated glass
DNA microarray
IL-6 receptor α
SV-T2 cells

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.06.083

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title = "Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass",

abstract = "We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.",

keywords = "BALB/c 3T3 cells, Cell surface marker, DLC-coated glass, DNA microarray, IL-6 receptor α, SV-T2 cells",

author = "Tuoya and Koichi Hirayama and Tadahiro Nagaoka and Dongwei Yu and Takayuki Fukuda and Hiroko Tada and Hidenori Yamada and Masaharu Seno",

note = "Funding Information: The authors thank Dr. Michifumi Tanga and Mr. Masayuki Kita for their continuous encouragement throughout the study. This study was supported by a Grant-in-Aid of the consortium for R&D projects for regional revitalization from METI. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2005",

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doi = "10.1016/j.bbrc.2005.06.083",

language = "English",

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pages = "263--268",

journal = "Biochemical and Biophysical Research Communications",

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AU - Tuoya,

AU - Hirayama, Koichi

AU - Nagaoka, Tadahiro

AU - Yu, Dongwei

AU - Fukuda, Takayuki

AU - Tada, Hiroko

AU - Yamada, Hidenori

AU - Seno, Masaharu

N1 - Funding Information: The authors thank Dr. Michifumi Tanga and Mr. Masayuki Kita for their continuous encouragement throughout the study. This study was supported by a Grant-in-Aid of the consortium for R&D projects for regional revitalization from METI. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2005/8/19

Y1 - 2005/8/19

N2 - We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

AB - We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor α gene expressions were upregulated in SV-T2 and were thought to be candidates for cell surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR, immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.

KW - BALB/c 3T3 cells

KW - Cell surface marker

KW - DLC-coated glass

KW - DNA microarray

KW - IL-6 receptor α

KW - SV-T2 cells

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Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass

Abstract

Keywords

ASJC Scopus subject areas

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