Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity

Shohei Domae, Yoichi Nakamura, Yurika Nakamura, Akiko Uenaka, Hisashi Wada, Masao Nakata, Mikio Oka, Koji Kishimoto, Goichi Tsukamoto, Yasuto Yoshihama, Junji Matsuoka, Akira Gochi, Shigeru Kohno, Takashi Saika, Akira Sasaki, Eiichi Nakayama, Toshiro Ono

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cancer/testis (CT) antigens are expressed in normal germ line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis-specific expression profiles in the Unigene database, including coiled-coil domain containing 62 (CCDC62). RT-PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62-2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62-2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62-2 was confirmed by western blot. CCDC62-2 is a CT antigen that is immunogenic in cancer patients.

Original languageEnglish
Pages (from-to)2347-2352
Number of pages6
JournalInternational Journal of Cancer
Volume124
Issue number10
DOIs
Publication statusPublished - May 15 2009

Keywords

  • Antibody response
  • CCDC62-2
  • Cancer/testis antigen
  • SEREX

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity'. Together they form a unique fingerprint.

  • Cite this

    Domae, S., Nakamura, Y., Nakamura, Y., Uenaka, A., Wada, H., Nakata, M., Oka, M., Kishimoto, K., Tsukamoto, G., Yoshihama, Y., Matsuoka, J., Gochi, A., Kohno, S., Saika, T., Sasaki, A., Nakayama, E., & Ono, T. (2009). Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity. International Journal of Cancer, 124(10), 2347-2352. https://doi.org/10.1002/ijc.24220