Abstract
The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.
| Original language | English |
|---|---|
| Pages (from-to) | 2000-2004 |
| Number of pages | 5 |
| Journal | Journal of Dental Research |
| Volume | 80 |
| Issue number | 11 |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Bactericidal activity
- Cacao bean husk
- Glucosyltransferase
- Polyphenol
- Streptococcus mutans
- Unsaturated fatty acid
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Identification of cariostatic substances in the cacao bean husk : Their anti-glucosyltransferase and antibacterial activities. / Osawa, K.; Miyazaki, K.; Shimura, S.; Okuda, J.; Nakano, Michiyo; Ooshima, T.
In: Journal of Dental Research, Vol. 80, No. 11, 2001, p. 2000-2004.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of cariostatic substances in the cacao bean husk
T2 - Their anti-glucosyltransferase and antibacterial activities
AU - Osawa, K.
AU - Miyazaki, K.
AU - Shimura, S.
AU - Okuda, J.
AU - Nakano, Michiyo
AU - Ooshima, T.
PY - 2001
Y1 - 2001
N2 - The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.
AB - The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.
KW - Bactericidal activity
KW - Cacao bean husk
KW - Glucosyltransferase
KW - Polyphenol
KW - Streptococcus mutans
KW - Unsaturated fatty acid
UR - http://www.scopus.com/inward/record.url?scp=0035734670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035734670&partnerID=8YFLogxK
M3 - Article
C2 - 11759010
AN - SCOPUS:0035734670
VL - 80
SP - 2000
EP - 2004
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 11
ER -