Identification of cariostatic substances in the cacao bean husk

Their anti-glucosyltransferase and antibacterial activities

K. Osawa, K. Miyazaki, S. Shimura, J. Okuda, Michiyo Nakano, T. Ooshima

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.

Original languageEnglish
Pages (from-to)2000-2004
Number of pages5
JournalJournal of Dental Research
Volume80
Issue number11
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Glucosyltransferases
Streptococcus mutans
Cacao
Molecular Weight
Oleic Acids
Linoleic Acids
Catechin
Dental Caries
Unsaturated Fatty Acids

Keywords

  • Bactericidal activity
  • Cacao bean husk
  • Glucosyltransferase
  • Polyphenol
  • Streptococcus mutans
  • Unsaturated fatty acid

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Identification of cariostatic substances in the cacao bean husk : Their anti-glucosyltransferase and antibacterial activities. / Osawa, K.; Miyazaki, K.; Shimura, S.; Okuda, J.; Nakano, Michiyo; Ooshima, T.

In: Journal of Dental Research, Vol. 80, No. 11, 2001, p. 2000-2004.

Research output: Contribution to journalArticle

Osawa, K. ; Miyazaki, K. ; Shimura, S. ; Okuda, J. ; Nakano, Michiyo ; Ooshima, T. / Identification of cariostatic substances in the cacao bean husk : Their anti-glucosyltransferase and antibacterial activities. In: Journal of Dental Research. 2001 ; Vol. 80, No. 11. pp. 2000-2004.
@article{40fc06f0c54f483d965e828294f889a5,
title = "Identification of cariostatic substances in the cacao bean husk: Their anti-glucosyltransferase and antibacterial activities",
abstract = "The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.",
keywords = "Bactericidal activity, Cacao bean husk, Glucosyltransferase, Polyphenol, Streptococcus mutans, Unsaturated fatty acid",
author = "K. Osawa and K. Miyazaki and S. Shimura and J. Okuda and Michiyo Nakano and T. Ooshima",
year = "2001",
language = "English",
volume = "80",
pages = "2000--2004",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "11",

}

TY - JOUR

T1 - Identification of cariostatic substances in the cacao bean husk

T2 - Their anti-glucosyltransferase and antibacterial activities

AU - Osawa, K.

AU - Miyazaki, K.

AU - Shimura, S.

AU - Okuda, J.

AU - Nakano, Michiyo

AU - Ooshima, T.

PY - 2001

Y1 - 2001

N2 - The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.

AB - The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed high-molecular-weight polyphenolic compounds and unsaturated fatty acids as active components. The former, which showed strong anti-GTF activity, were polymeric epicatechins with C-4β and C-8 intermolecular bonds estimated to be 4636 in molecular weight in an acetylated form. The latter, which showed bactericidal activity against Streptococcus mutans, were determined to be oleic and linoleic acids, and demonstrated a high level of activity at a concentration of 30 μg/mL. The cariostatic activity of the cacao bean husk is likely caused by these biologically active constituents.

KW - Bactericidal activity

KW - Cacao bean husk

KW - Glucosyltransferase

KW - Polyphenol

KW - Streptococcus mutans

KW - Unsaturated fatty acid

UR - http://www.scopus.com/inward/record.url?scp=0035734670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035734670&partnerID=8YFLogxK

M3 - Article

VL - 80

SP - 2000

EP - 2004

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 11

ER -