Identification of an RNA silencing suppressor encoded by a symptomless fungal hypovirus, Cryphonectria hypovirus 4

Annisa Aulia, Kiwamu Hyodo, Sakae Hisano, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

Previously, we have reported the ability of a symptomless hypovirus Cryphonectria hypovirus 4 (CHV4) of the chestnut blight fungus to facilitate stable infection by a co-infecting mycoreovirus 2 (MyRV2)—likely through the inhibitory effect of CHV4 on RNA silencing (Aulia et al., Virology, 2019). In this study, the N-terminal portion of the CHV4 polyprotein, termed p24, is identified as an autocatalytic protease capable of suppressing host antiviral RNA silencing. Using a bacterial expression system, CHV4 p24 is shown to cleave autocatalytically at the di-glycine peptide (Gly214-Gly215) of the polyprotein through its protease activity. Transgenic expression of CHV4 p24 in Cryphonectria parasitica suppresses the induction of one of the key genes of the antiviral RNA silencing, dicer-like 2, and stabilizes the infection of RNA silencing-susceptible virus MyRV2. This study shows functional similarity between CHV4 p24 and its homolog p29, encoded by the symptomatic prototype hypovirus CHV1.

Original languageEnglish
Article number100
Pages (from-to)1-16
Number of pages16
JournalBiology
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2021
Externally publishedYes

Keywords

  • Chestnut blight fungus
  • Co-infection
  • Cryphonectria parasitica
  • Dicer
  • Hypovirus
  • Mycovirus
  • RNA silencing
  • RNAi suppressor
  • Reovirus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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