Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

Y. Hayashizaki, H. Shibata, S. Hirotsune, H. Sugino, Y. Okazaki, N. Sasaki, K. Hirose, H. Imoto, H. Okuizumi, M. Muramatsu, H. Komatsubara, T. Shiroishi, K. Moriwaki, M. Katsuki, N. Hatano, H. Sasaki, T. Ueda, N. Mise, N. Takagi, C. PlassV. M. Chapman

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190 Citations (Scopus)

Abstract

A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain–specific Notl and BssHII spots were identified as imprinted in reciprocal F1 hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT–PCR of reciprocal F1–hybrid mice. The amino–acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalNature Genetics
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 1994
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Hayashizaki, Y., Shibata, H., Hirotsune, S., Sugino, H., Okazaki, Y., Sasaki, N., Hirose, K., Imoto, H., Okuizumi, H., Muramatsu, M., Komatsubara, H., Shiroishi, T., Moriwaki, K., Katsuki, M., Hatano, N., Sasaki, H., Ueda, T., Mise, N., Takagi, N., ... Chapman, V. M. (1994). Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method. Nature Genetics, 6(1), 33-40. https://doi.org/10.1038/ng0194-33