Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport

Jiye Rhee, Tomoaki Horie, Shizuka Sasano, Yoshiki Nakahara, Maki Katsuhara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H2O2 permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H2O2-transporting aquaporins among 15 barley and rice TIPs. Influx of H2O2 into yeast cells expressing HvPIP2;5 was determined with a florescent-dye-based assay. Indirect immunofluorescence indicated that the expression of HvPIP2;5 protein was ubiquitous in root tissues, and was also weakly observed in leaf epidermal cells and cells in the vascular bundle. Point mutated variants of HvPIP2;5 were generated by the site-directed mutagenesis. Growth assays of yeast cells expressing these mutated HvPIP2;5 proteins suggested that Ser-126 in HvPIP2;5 has a large impact on H2O2 transport with a minor influence on the HvPIP2;5-mediated water transport.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalPhysiologia Plantarum
Volume159
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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