Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport

Jiye Rhee, Tomoaki Horie, Shizuka Sasano, Yoshiki Nakahara, Maki Katsuhara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H2O2 permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H2O2-transporting aquaporins among 15 barley and rice TIPs. Influx of H2O2 into yeast cells expressing HvPIP2;5 was determined with a florescent-dye-based assay. Indirect immunofluorescence indicated that the expression of HvPIP2;5 protein was ubiquitous in root tissues, and was also weakly observed in leaf epidermal cells and cells in the vascular bundle. Point mutated variants of HvPIP2;5 were generated by the site-directed mutagenesis. Growth assays of yeast cells expressing these mutated HvPIP2;5 proteins suggested that Ser-126 in HvPIP2;5 has a large impact on H2O2 transport with a minor influence on the HvPIP2;5-mediated water transport.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalPhysiologia Plantarum
Volume159
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

plant-incorporated protectants
Aquaporins
aquaporins
Hordeum
serine
Serine
barley
Aquaporin 5
Yeasts
cells
yeasts
rice
site-directed mutagenesis
vascular bundles
assays
Indirect Fluorescent Antibody Technique
Site-Directed Mutagenesis
Hordeum vulgare
fluorescent antibody technique
Blood Vessels

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport. / Rhee, Jiye; Horie, Tomoaki; Sasano, Shizuka; Nakahara, Yoshiki; Katsuhara, Maki.

In: Physiologia Plantarum, Vol. 159, No. 1, 01.01.2017, p. 120-128.

Research output: Contribution to journalArticle

Rhee, Jiye ; Horie, Tomoaki ; Sasano, Shizuka ; Nakahara, Yoshiki ; Katsuhara, Maki. / Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport. In: Physiologia Plantarum. 2017 ; Vol. 159, No. 1. pp. 120-128.
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