Identification of an HLA-A24-restricted OY-TES-1 epitope recognized by cytotoxic T-cells

Hideo Okumura, Yuji Noguchi, Akiko Uenaka, Toshiki Aji, Toshiro Ono, Kazuhiko Nakagawa, Motoi Aoe, Nobuyoshi Shimizu, Eiichi Nakayama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

OY-TES-1 was identified as a human homologue of the mouse, guinea pig, and pig proacrosin binding protein sp32 precursor. Differential expression levels of OY-TES-1 mRNA between testis and other normal tissues, and its expression in cancers indicated that OY-TES-1 would be classified as a cancer/testis antigen and considered to be a candidate of target antigen for cancer immunotherapy. In this study, we showed identification of HLA-A24-binding OY-TES-1 peptide, TES401-409, (KTPFVSPLL) recognized by CD8 T-cells. Purified CD8 T-cells from healthy donors stimulated in vitro with the peptide-pulsed autologous DC and PBMC produced IFNγ in response to the peptide-pulsed PBMC and showed cytotoxicity against the peptide-pulsed autologous EBV-B specifically. Furthermore, cytotoxicity was also observed against an OY-TES-1 mRNA-expressing tumor line, LK79. The retention time of the fraction in HPLC of the acid eluate from LK79 cells that showed positive sensitization against autologous EBV-B cells in recognition by CD8 CTL was the same as that of the fraction of the TES401-409 peptide itself, suggesting that the TES401-409 was a naturally processed peptide on LK79.

Original languageEnglish
Pages (from-to)1009-1016
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume49
Issue number11
DOIs
Publication statusPublished - 2005

Keywords

  • Acid extraction
  • CTL epitope
  • Cancer/testis antigen
  • OY-TES-1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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  • Cite this

    Okumura, H., Noguchi, Y., Uenaka, A., Aji, T., Ono, T., Nakagawa, K., Aoe, M., Shimizu, N., & Nakayama, E. (2005). Identification of an HLA-A24-restricted OY-TES-1 epitope recognized by cytotoxic T-cells. MICROBIOLOGY and IMMUNOLOGY, 49(11), 1009-1016. https://doi.org/10.1111/j.1348-0421.2005.tb03688.x