Identification of an allele of VAM3/SYP22 that confers a semi-dwarf phenotype in Arabidopsis thaliana

Ichiro Ohtomo, Haruko Ueda, Tomoo Shimada, Chiaki Nishiyama, Yasuko Komoto, Ikuko Hara-Nishimura, Taku Takahashi

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

The short stem and midrib (ssm) mutants of Arabidopsis thaliana show both semi-dwarf and wavy leaf phenotypes due to defects in the elongation of the stem internodes and leaves. Moreover, these abnormalities cannot be recovered by exogenous phytohormones. ssm was originally identified as a single recessive mutant of the ecotype Columbia (Col-0), but genetic crossing experiments have revealed that this mutant phenotype is restored by another gene that is functional in the ecotype Landsberg erecta (Ler) and not in Col-0. Map-based cloning of the gene that is defective in ssm mutants has uncovered a small deletion in the sixth intron of a gene encoding a syntaxin, VAM3/SYP22, which has been implicated in vesicle transport to the vacuole. This mutation appears to cause a peptide insertion in the deduced VAM3/SYP22 polypeptide sequence due to defective splicing of the shortened sixth intron. Significantly, when compared with the wild-type Ler genome, the wildtype Col-0 genome has a single base pair deletion causing a frameshift mutation in SYP23, a gene with the highest known homology to VAM3/SYP22. These findings suggest that VAM3/SYP22 and SYP23 have overlapping functions and that the vesicle transport mediated by these syntaxins is important for shoot morphogenesis.

Original languageEnglish
Pages (from-to)1358-1365
Number of pages8
JournalPlant and Cell Physiology
Volume46
Issue number8
DOIs
Publication statusPublished - Aug 2005

Keywords

  • Arabidopsis
  • Cell elongation
  • Dwarf
  • Syntaxin
  • VAM3

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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