Identification of amino acids in auxiliary replicase protein p27 critical for its RNA-binding activity and the assembly of the replicase complex in Red clover necrotic mosaic virus

Kiwamu Hyodo, Akira Mine, Hiro oki Iwakawa, Masanori Kaido, Kazuyuki Mise, Tetsuro Okuno

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The specific recognition of genomic RNAs by viral replicase proteins is a key regulatory step during the early replication process in positive-strand RNA viruses. In this study, we characterized the RNA-binding activity of the auxiliary replicase protein p27 of Red clover necrotic mosaic virus (RCNMV), which has a bipartite genome consisting of RNA1 and RNA2. Aptamer pull-down assays identified the amino acid residues of p27 involved in its specific interaction with RNA2. The RNA-binding activity of p27 correlated with its activity in recruiting RNA2 to membranes. We also identified the amino acids required for the formation of the 480-kDa replicase complex, a key player of RCNMV RNA replication. These amino acids are not involved in the functions of p27 that bind viral RNA or replicase proteins, suggesting an additional role for p27 in the assembly of the replicase complex. Our results demonstrate that p27 has multiple functions in RCNMV replication.

Original languageEnglish
Pages (from-to)300-309
Number of pages10
JournalVirology
Volume413
Issue number2
DOIs
Publication statusPublished - May 10 2011
Externally publishedYes

Keywords

  • Dianthovirus
  • Positive-strand RNA virus
  • RNA recognition
  • RNA replication
  • RNA-binding
  • Red clover necrotic mosaic virus
  • Replicase complex
  • Replicase proteins

ASJC Scopus subject areas

  • Virology

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